Mucin-1-Antibody-Conjugated Mesoporous Silica Nanoparticles for Selective Breast Cancer Detection in a Mucin-1 Transgenic Murine Mouse Model.
Mucin-1-Antibody-Conjugated Mesoporous Silica Nanoparticles for Selective Breast Cancer Detection in a Mucin-1 Transgenic Murine Mouse Model.
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DOI：
10.1166/jbn.2016.2318
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发表时间：
2016-12
影响因子：
2.9
通讯作者：
Vivero-Escoto JL
中科院分区：
文献类型：
--
作者：
Dréau D;Moore LJ;Alvarez-Berrios MP;Tarannum M;Mukherjee P;Vivero-Escoto JL
粘蛋白-1(MUC1)是一种跨膜糖蛋白，在~90%的乳腺癌组织中异常表达，是纳米粒子靶向成像的理想靶点。在这项研究中，报道了一种用于体内光学检测乳腺癌组织的染料掺杂的介孔二氧化硅纳米颗粒平台(ab-tMUC1-NIR-MSN)的研制。对该纳米粒子探针的结构性质、体内外性能进行了评价。体外研究表明，基于MSN的光学成像纳米探针是无细胞毒性的，并且有效地靶向过表达人tMUC1蛋白的乳腺癌细胞。用雌性C57BL/6小鼠进行的体内实验表明，该平台主要在肝脏内蓄积，不会产生短期毒性。此外，我们证明了ab-tMUC1-NIR-MSN纳米探针在人MUC1转基因小鼠模型中特异性地检测到过表达人tMUC1的乳腺肿瘤。
Mucin-1 (MUC1), a transmembrane glycoprotein is aberrantly expressed on ~90% of breast cancer and is an excellent target for nanoparticulate targeted imaging. In this study, the development of a dye-doped NIR emitting mesoporous silica nanoparticles platform conjugated to tumor-specific MUC1 antibody (ab-tMUC1-NIR-MSN) for in vivo optical detection of breast adenocarcinoma tissue is reported. The structural properties, the in vitro and in vivo performance of this nanoparticle-based probe were evaluated. In vitro studies showed that the MSN-based optical imaging nanoprobe is non-cytotoxic and targets efficiently mammary cancer cells overexpressing human tMUC1 protein. In vivo experiments with female C57BL/6 mice indicated that this platform accumulates mainly in the liver and did not induce short-term toxicity. In addition, we demonstrated that the ab-tMUC1-NIR-MSN nanoprobe specifically detects mammary gland tumors overexpressing human tMUC1 in a human MUC1 transgenic mouse model.