RAPID DETECTION AND IDENTIFICATION OF JC-VIRUS AND BK-VIRUS IN HUMAN-URINE BY USING IMMUNOFLUORESCENCE MICROSCOPY
RAPID DETECTION AND IDENTIFICATION OF JC-VIRUS AND BK-VIRUS IN HUMAN-URINE BY USING IMMUNOFLUORESCENCE MICROSCOPY
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DOI：
10.1128/jcm.11.2.178-183.1980
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发表时间：
1980-01-01
影响因子：
9.4
通讯作者：
MCBAIN, JA
中科院分区：
文献类型：
--
作者：
HOGAN, TF;PADGETT, BL;MCBAIN, JA
建立了间接免疫荧光法，并用于检测JC病毒（JCV）和BK病毒（BKV）感染的异常移行细胞尿排泄物。并与尿细胞学、电镜、病毒培养及血清学等方法对肾移植受者及正常对照组进行比较。间接免疫荧光法检测出4例JCV排泄，1例BKV排泄，8例JCV和BKV同时排泄。仅在细胞学异常细胞的细胞核中发现病毒抗原。在这13人中，有8人还通过EM在尿液中检测到多瘤病毒体。通过对连续尿样的重复研究，30%的移植受者和6%的正常对照者通过一种或多种显微镜方法呈阳性。血清学结果证实，在免疫抑制的患者中，JCV和BKV增殖的发生率很高。血清学与尿病毒学结果无直接相关性。尿细胞学检查和间接免疫荧光法是检测和鉴定尿中JCV和BKV的快速、敏感的方法。
An indirect immunofluorescence method was developed and used to detect urinary excretion of abnormal transitional cells infected with JC virus (JCV) or BK virus (BKV). This method was compared with urinary cytology, EM, viral culture and viral serology in groups of immunosuppressed renal transplant recipients and normal controls. The indirect immunofluorescence method detected and identified JCV excretion in 4 persons, BKV excretion in 1 person and both JCV and BKV excretion in 8 others. Viral antigen was identified only in the nuclei of cytologically abnormal cells. Of these 13 persons, 8 also had polyoma virions detected in the urine by EM. With repeated study of sequential urine samples, 30% of transplant recipients and 6% of normal controls were positive by 1 or more microscopy methods. Serological results confirmed a high incidence of JCV and BKV multiplication in the immunosuppressed patients. Serology did not correlate directly with urinary virological findings. Urinary cytology and the indirect immunofluorescence method were rapid and sensitive methods for detecting and identifying urinary excretion of JCV and BKV.