Endothelium-dependent relaxation is depressed at the macro- and microcirculatory levels during sepsis.
Endothelium-dependent relaxation is depressed at the macro- and microcirculatory levels during sepsis.
复制标题
脓毒症期间，宏观和微循环水平上的内皮依赖性舒张受到抑制。
DOI：
10.1152/ajpregu.1995.269.5.r988
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chaudry,IH
中科院分区：
文献类型：
--
作者：
Wang,P;Ba,ZF;Chaudry,IH
本研究的目的是确定是否内皮源性一氧化氮（NO）的生产减少在大循环和微循环水平在败血症。为了检验这一点，通过盲肠结扎和穿孔（CLP）使大鼠经受脓毒症。CLP后5 h（即，高动力败血症中点）或假手术时，分离主动脉和上级肠系膜动脉。对内皮依赖性血管扩张剂乙酰胆碱（ACh）和内皮非依赖性血管扩张剂硝酸甘油（NTG）的反应进行了测定。在其他研究中，在CLP后5或20小时（低动力性脓毒症）分离小肠。在离体肠中测定对ACh和NTG的反应。结果表明，CLP后5小时，主动脉和上级肠系膜动脉的内皮依赖性舒张功能均受到抑制。与此相反，NTG引起的舒张作用无显著差异。此外，乙酰胆碱诱导的血管舒张在离体小肠CLP后5和20小时下降，而没有任何显着改变NTG诱导的松弛。由于研究表明，ACh诱导的舒张主动脉在CLP后20小时减少，可以得出结论，内皮源性NO释放在脓毒症的高动力和低动力阶段受到抑制，不仅在大动脉，而且在微循环水平。
The objective of this study was to determine whether endothelium-derived nitric oxide (NO) production is reduced at the macrocirculatory and microcirculatory levels during sepsis. To examine this, rats were subjected to sepsis by cecal ligation and puncture (CLP). At 5 h after CLP (i.e., midpoint of hyperdynamic sepsis) or sham operation, the aorta and superior mesenteric artery were isolated. Responses to an endothelium-dependent vasodilator, acetylcholine (ACh), and an endothelium-independent vasodilator, nitroglycerin (NTG), were determined. In additional studies, the small intestine was isolated 5 or 20 h (hypodynamic sepsis) after CLP. Responses to ACh and NTG were determined in the isolated intestine. The results indicate that endothelium-dependent relaxation in both the aorta and superior mesenteric artery was depressed at 5 h after CLP. In contrast, there was no significant difference in the relaxation induced by NTG. Moreover, ACh-induced vascular relaxation in the isolated small intestine decreased at 5 and 20 h post-CLP without any significant alterations in NTG-induced relaxation. Since studies have shown that ACh-induced relaxation in the aorta is reduced at 20 h after CLP, it could be concluded that endothelium-derived NO release is depressed during hyperdynamic and hypodynamic stages of sepsis, not only in large arteries, but also at the microcirculatory level.