Alterations in peripheral blood lymphocyte cytokine expression in obesity
Alterations in peripheral blood lymphocyte cytokine expression in obesity
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DOI：
10.1111/j.1365-2249.2006.03186.x
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发表时间：
2006-10-01
影响因子：
4.6
通讯作者：
Rosenbaum, J. T.
中科院分区：
文献类型：
--
作者：
O'Rourke, R. W.;Kay, T.;Rosenbaum, J. T.
肥胖的特征是免疫和炎症功能的改变。为了评估外周血单个核细胞（PBMC）表达细胞因子在肥胖相关炎症中的潜在作用，我们研究了9名瘦型和10名肥胖受试者的血清蛋白水平和PBMC中白细胞介素（IL）-6，IL-1 β，肿瘤坏死因子（TNF）-α和IL-1 Ra的mRNA水平。与瘦型受试者相比，肥胖受试者的血清IL-1 β检测不到，IL-1 Ra血清水平升高，TNF-α血清水平降低，IL-6血清水平相似，而肥胖受试者PBMC中IL-6、IL-1 β和TNF-α的转录水平降低，但IL-1 Ra没有降低。然而，来自肥胖受试者的PBMC确实上调细胞因子表达以响应瘦素。因此，肥胖相关的IL-1 Ra血清水平和IL-6 mRNA水平的变化分别与同源mRNA和血清水平的变化无关，而肥胖患者的TNF-α血清水平和PBMC mRNA水平均降低。虽然肥胖中的免疫改变在外周血淋巴细胞中是明显的，但是改变的血清水平和改变的PBMC基因表达之间通常缺乏相关性，这表明PBMC可能不是肥胖中异常血清细胞因子水平的来源。
Obesity is characterized by alterations in immune and inflammatory function. In order to evaluate the potential role of cytokine expression by peripheral blood mononuclear cells (PBMC) in obesity-associated inflammation, we studied serum protein levels and mRNA levels in PBMC of interleukin (IL)-6, IL-1 beta, tumour necrosis factor (TNF)-alpha and IL-1Ra in nine lean and 10 obese subjects. Serum IL-1 beta was undetectable, IL-1Ra serum levels were elevated, serum levels of TNF-alpha were decreased and serum levels of IL-6 were similar in obese subjects compared to lean subjects, while transcript levels of IL-6, IL-1 beta and TNF-alpha, but not IL-1Ra, were decreased in PBMC from obese subjects. PBMC from obese subjects did, however, up-regulate cytokine expression in response to leptin. Thus, obesity-associated changes in IL-1Ra serum levels and IL-6 mRNA levels were not correlated with changes in cognate mRNA and serum levels, respectively, while TNF-alpha serum levels and PBMC mRNA levels were both decreased in obese patients. While immune alterations in obesity are manifest in peripheral blood lymphocytes, the general lack of correlation between altered serum levels and altered PBMC gene expression suggests that PBMC may not be the source of aberrant serum cytokine levels in obesity.