Association of RAD 51 135 G/C, 172 G/T and XRCC3 Thr241Met Gene Polymorphisms with Increased Risk of Head and Neck Cancer
Association of RAD 51 135 G/C, 172 G/T and XRCC3 Thr241Met Gene Polymorphisms with Increased Risk of Head and Neck Cancer
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DOI：
10.7314/apjcp.2014.15.23.10457
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Mahjabeen, Ishrat
中科院分区：
文献类型：
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作者：
Kayani, Mahmood Akhtar;Khan, Sumeera;Mahjabeen, Ishrat
同源重组修复(HRR)在对抗致癌因子方面发挥着重要作用。调节HRR机制的基因可能会损害它们的功能，从而导致癌症易感性增加。RAD 51和XRCC3是HRR途径的关键调节因子，它们的遗传变异可能导致包括头颈癌(HNC)在内的各种癌症的出现和发展。本研究的目的是比较HNC患者和对照组RAD51(135G/C，172G/T)和XRCC3(Thr241Met)基因多态性的分布。用聚合酶链式反应-限制性片段长度聚合酶(PCR-RFLP)技术对200例经病理证实的HNC患者和150例正常、无疾病的健康人的血液样本进行了基因分型。我们观察到RAD51 135G/C纯合子变异CC基因型与HNC风险增加2.5倍相关(OR=2.5；95%CI=0.69-9.53；p
Homologous recombination repair (HRR) plays an important role in protection against carcinogenic factors. Genes regulating the HRR mechanisms may impair their functions and consequently result in increased cancer susceptibility. RAD 51 and XRCC3 are key regulators of the HRR pathway and genetic variability in these may contribute to the appearance and progression of various cancers including head and neck cancer (HNC). The aim of the present study was to compare the distribution of genotypes of RAD51 (135G/C, 172 G/T) and XRCC3 (Thr241Met) polymorphisms between HNC patients and controls. Each polymorphism was genotyped using the polymerase chain reaction-restriction fragment length polymerase (PCR-RFLP) technique in 200 pathologically confirmed HNC patients along with 150 blood samples from normal, disease free healthy individuals. We observed that homozygous variant CC genotype of RAD51 135G/C was associated with a 2.5 fold increased HNC risk (OR=2.5; 95% CI=0.69-9.53; p