Characterization and biosynthesis of proteoglycans of corneal stroma from rhesus monkey.
Characterization and biosynthesis of proteoglycans of corneal stroma from rhesus monkey.
复制标题
恒河猴角膜基质蛋白多糖的表征和生物合成。
DOI：
10.1016/s0021-9258(19)86322-6
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
V. Hascall
中科院分区：
文献类型：
--
作者：
J. Hassell;D. Newsome;V. Hascall
通过分析器官培养中角膜合成的放射性标记蛋白聚糖和大规模制备获得的天然角膜蛋白聚糖，对恒河猴角膜基质的蛋白聚糖进行表征。分析表明，在器官培养物中合成的蛋白聚糖是相似的，如果不是相同的，他们在基质中的对应物，虽然他们在体外合成的比例不同，比他们在体内积累。角膜基质含有两种蛋白多糖。软骨素-硫酸皮肤素蛋白聚糖由约70%的蛋白质组成，并且具有Mr =约100，000至150，000。它含有一个Mr =约55，000的硫酸软骨素-皮肤素侧链。硫酸角质素蛋白聚糖由约74%的蛋白质组成，并且具有约-40，000至70，000的Mr。它含有一个或两个硫酸角质素侧链，每个侧链的Mr =约7，000。放射性标记表明，这两种蛋白聚糖含有糖蛋白型寡糖作为其结构的一部分。
The proteoglycans of the Rhesus monkey corneal stroma were characterized by analyzing both radiolabeled proteoglycans synthesized by corneas in organ culture and native corneal proteoglycans obtained by large scale preparations. The analyses indicate that the proteoglycans synthesized in organ culture were similar to, if not identical with, their counterparts in the stroma although they are synthesized in different prportions in vitro than they acumulate in vivo. The corneal stroma contains two proteoglycans. The chondroitin-dermatan sulfate proteoglycan consists of approximately 70% protein and has a Mr = approximately 100,000 to 150,000. It contains one chondroitin-dermatan sulfate side chain of Mr = approximately 55,000. The keratan sulfate proteoglycan consists of approximately 74% protein and has a Mr = approximately -40,000 to 70,000. It contains one or two keratan sulfate side chains with a Mr = approximately 7,000 each. Radiolabeling indicates that both proteoglycans contain glycoprotein-type oligosaccharides as part of their structure.