Biological effects of cigarette smoke in cultured human retinal pigment epithelial cells.
Biological effects of cigarette smoke in cultured human retinal pigment epithelial cells.
复制标题
香烟烟雾在培养的人视网膜色素上皮细胞中的生物学作用。
DOI：
10.1371/journal.pone.0048501
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Welge-Lussen U
中科院分区：
文献类型：
--
作者：
Yu AL;Birke K;Burger J;Welge-Lussen U
本研究的目的是确定香烟烟雾提取物 (CSE) 处理是否会诱导原代人视网膜色素上皮 (RPE) 细胞的细胞损失、细胞衰老和细胞外基质 (ECM) 合成。原代培养的人 RPE 细胞暴露于 2%、4%、8% 和 12% 的 CSE 浓度下 24 小时。通过细胞活力测定检测细胞损失。通过顺式石蜡油酸 (PNA) 荧光的丧失来评估脂质过氧化。通过组织化学染色检测衰老相关的β-半乳糖苷酶（SA-β-Gal）活性。通过实时 PCR、蛋白质印迹或 ELISA 实验检查载脂蛋白 J (Apo J)、结缔组织生长因子 (CTGF)、纤连蛋白和层粘连蛋白的表达。结果表明，将细胞暴露于 12% 的 CSE 浓度会诱导细胞死亡，而用 2%、4% 和 8% CSE 处理细胞会增加脂质过氧化。暴露于 8% 的 CSE 中，SA-ß-Gal 阳性细胞的数量显着增加至 82%，Apo J、CTGF 和纤连蛋白的 mRNA 表达增加约 3-4 倍。 8% CSE 处理还增加了 Apo J 和 CTGF 的蛋白表达以及纤连蛋白和层粘连蛋白的分泌。因此，CSE 治疗可诱导原代人 RPE 细胞的细胞损失、衰老变化和 ECM 合成。可以推测，香烟烟雾可能参与 RPE 细胞的细胞事件，如年龄相关性黄斑变性中所见。
The goal of the present study was to determine whether treatment with cigarette smoke extract (CSE) induces cell loss, cellular senescence, and extracellular matrix (ECM) synthesis in primary human retinal pigment epithelial (RPE) cells. Primary cultured human RPE cells were exposed to 2, 4, 8, and 12% of CSE concentration for 24 hours. Cell loss was detected by cell viability assay. Lipid peroxidation was assessed by loss of cis-parinaric acid (PNA) fluorescence. Senescence-associated ß-galactosidase (SA-ß-Gal) activity was detected by histochemical staining. Expression of apolipoprotein J (Apo J), connective tissue growth factor (CTGF), fibronectin, and laminin were examined by real-time PCR, western blot, or ELISA experiments. The results showed that exposure of cells to 12% of CSE concentration induced cell death, while treatment of cells with 2, 4, and 8% CSE increased lipid peroxidation. Exposure to 8% of CSE markedly increased the number of SA-ß-Gal positive cells to up to 82%, and the mRNA expression of Apo J, CTGF, and fibronectin by approximately 3–4 fold. Treatment with 8% of CSE also increased the protein expression of Apo J and CTGF and the secretion of fibronectin and laminin. Thus, treatment with CSE can induce cell loss, senescent changes, and ECM synthesis in primary human RPE cells. It may be speculated that cigarette smoke could be involved in cellular events in RPE cells as seen in age-related macular degeneration.
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影响因子：
3.7
作者：
Fujihara, Masashi;Nagai, Norihiro;Sussan, Thomas E.;Biswal, Shyam;Handa, James T.
通讯作者：
Handa, James T.
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
4.8
作者：
Bernhard, D;Pfister, G;Wick, G
通讯作者：
Wick, G
DOI：
10.1152/ajplung.90215.2008
发表时间：
2008-10-01
影响因子：
4.9
作者：
Baglole, Carolyn J.;Sime, Patricia J.;Phipps, Richard P.
通讯作者：
Phipps, Richard P.
影响因子：
3.4
作者：
Fuchshofer, Rudolf;Yu, Alice L.;Welge-Lussen, Ulrich
通讯作者：
Welge-Lussen, Ulrich