Genotyping of two congeneric bitterling fish species by nuclear SNP markers and the detection of hybridization in a sympatric region
Genotyping of two congeneric bitterling fish species by nuclear SNP markers and the detection of hybridization in a sympatric region
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DOI：
10.1111/1440-1703.12387
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发表时间：
2023-02
影响因子：
2
通讯作者：
H. Hata;Rintaro Taniguchi;Naoki Yamashita;Y. Hashiguchi;Jun Nakajima;Tomohiro Takeyama
中科院分区：
文献类型：
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作者：
H. Hata;Rintaro Taniguchi;Naoki Yamashita;Y. Hashiguchi;Jun Nakajima;Tomohiro Takeyama
苦味鱼类（亚科：Acheilognathinae）在日本由于栖息地的丧失而濒临灭绝。淡水贻贝的减少也加剧了幼贝的减少，因为幼贝在淡水贻贝的鳃中产卵。此外，蚌类的损失导致了蚌类物种之间的杂交。本研究的目的是分析在一个保存完好的地点，两个物种自然发生的同源物种之间的杂交频率，并将其与其他受人类干预影响更严重的地区进行比较。冈山是朝日水系灌溉河流的一部分，居住着日本的一种天然纪念物种，一种底栖鳕鱼Parabotia curtus，因此，天然底层和结构异质的河岸得以保持。我们在这个保存完好的地点收集了Tanakiaindivisions并调查了贻贝密度。我们还在不同的染色体上开发了六个核单核苷酸多态性（SNP）标记来进行BSCT。lanceolata和T.林巴塔这些SNP标记有效地确定个体是这些物种之一还是杂交种。根据基因分型，8个个体被确定为杂种，而90和173个个体被确定为T的纯种。lanceolata和T.林巴塔，分别。杂交种的比例为3.0%，与其他地区相比相对较低。此外，有5种蚌类在该溪流中密集分布。同域T. lanceolata和T. limbata是在这个保存完好的地方建立的，那里有密集的蚌类，为这两个物种提供了足够的繁殖基质。
Bitterling fish species (subfamily: Acheilognathinae) are endangered in Japan due to habitat loss. The decline of freshwater mussels also enhances the decrease of bitterlings because bitterlings lay eggs in the gills of freshwater mussels. Furthermore, loss of unionid mussels causes hybridization between bitterling species. This study aims to analyze the frequency of hybridization between congeneric species of bitterlings—Tanakia lanceolataandTanakia limbata—at a well‐preserved site, where both species occur naturally and compare this with other regions more severely impacted by human intervention. One part of the irrigation streams of the Asahi River System, Okayama, is inhabited by a natural monument species of Japan, a benthic cobitid,Parabotia curtus, and therefore, the natural substratum and the structurally heterogeneous stream banks are maintained. We collectedTanakiaindividuals and surveyed mussel density at this well‐preserved site. We also developed six nuclear single‐nucleotide polymorphism (SNP) markers on different chromosomes to distinguishT. lanceolataandT. limbata. These SNP markers effectively determined individuals as being either one of these species or a hybrid. Based on genotyping, eight individuals were determined to be hybrids, whereas 90 and 173 individuals were determined as purebreds ofT. lanceolataandT. limbata, respectively. The proportion of hybrid individuals was 3.0% and was relatively low compared to other regions. In addition, five species of unionids were densely distributed in this stream. Prezygotic isolation between sympatricT. lanceolataandT. limbatawas established at this well‐preserved site, where unionid mussels are densely populated and provide enough breeding substrate for both species.