Free Radical Polymerization of Gold Nanoclusters and Hydrogels for Cell Capture and Light-Controlled Release.
Free Radical Polymerization of Gold Nanoclusters and Hydrogels for Cell Capture and Light-Controlled Release.
复制标题
DOI：
10.1021/acsami.1c03587
复制
发表时间：
2021-04
影响因子：
9.5
通讯作者：
Shuxian Zhu;Xiaoyu Wang;Y. Cong;Lu Liu;Lidong Li
中科院分区：
文献类型：
--
作者：
Shuxian Zhu;Xiaoyu Wang;Y. Cong;Lu Liu;Lidong Li
金纳米团簇修饰的水凝胶作为一种多用途的生物材料引起了人们的广泛关注。迄今为止，AuNC和水凝胶主要作为独立组分混合。在这里，我们报告的使用AuNC作为反应性单体在水凝胶的聚合。我们采用自由基聚合法将金纳米粒子与丙烯酰胺和N-丙烯酰甘氨酰胺共聚，制备了刺激响应性智能水凝胶。多个C → C键被修饰在AuNCs的表面上作为聚合的活性位点。这些C → C键不仅保护了AuNCs的结构在聚合过程中不被自由基氧化，而且还将AuNCs与聚合物链共价连接。这种结构确保了AuNCs的良好光热性能，同时保留了聚合物的温敏氢键。此外，共聚金纳米碳作为交联剂，这提高了水凝胶的机械性能。这些智能水凝胶具有良好的稳定性、高效的光热转换和灵敏的温敏性。我们研究了它们以透明质酸为靶分子捕获MDA-MB-231细胞的潜力。捕获的细胞在660 nm照射下释放。这种有针对性的捕获和光控遥控释放的过程可以重复应用。这些结果表明，基于AuNCs与水凝胶共聚的系统具有巨大的生物医学应用潜力。
Gold nanocluster (AuNC) decorated hydrogels have attracted considerable attention as versatile biomaterials. To date AuNCs and hydrogels have mainly been mixed as independent components. Here, we report the use of AuNCs as reactive monomers in the polymerization of hydrogels. We used a free radical polymerization to copolymerize AuNCs with acrylamide and N-acryloyl glycinamide to prepare stimuli-responsive smart hydrogels. Multiple C═C bonds were decorated on the surface of the AuNCs as active sites for polymerization. These C═C bonds not only protected the structure of the AuNCs from oxidation by free radicals during polymerization but also covalently connected the AuNCs with the polymer chains. This structure ensured good photothermal performance of the AuNCs while preserving the thermoresponsive hydrogen bonds of polymers. Moreover, the copolymerized AuNCs acted as cross-linkers, which improved the mechanical properties of the hydrogels. These smart hydrogels had good stability, efficient photothermal conversion, and a sensitive thermoresponsive. We examined their potential for capture of MDA-MB-231 cells with hyaluronic acid as target molecules. The captured cells were released under 660 nm irradiation. This process of targeted capture and light-controlled remote release could be repeatedly applied. These results suggest that systems based on AuNCs copolymerized with hydrogels have great potential for biomedical applications.