EXPRESSION OF SURROGATE LIGHT CHAIN RECEPTORS IS RESTRICTED TO A LATE STAGE IN PRE-B CELL-DIFFERENTIATION
EXPRESSION OF SURROGATE LIGHT CHAIN RECEPTORS IS RESTRICTED TO A LATE STAGE IN PRE-B CELL-DIFFERENTIATION
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DOI：
10.1016/0092-8674(93)90161-i
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发表时间：
1993-04-09
期刊：
影响因子：
64.5
通讯作者：
COOPER, MD
中科院分区：
文献类型：
--
作者：
LASSOUED, K;NUNEZ, CA;COOPER, MD
在免疫球蛋白基因重排之前，替代轻链（psiLC）基因在祖细胞B （pro-B）细胞中具有转录活性。目前的假设模型表明，psiLC蛋白可能与替代或传统的重链蛋白偶联，形成细胞表面受体，指示前B、前体B和未成熟B细胞的逐渐分化。制备单克隆抗体，用于检测B细胞分化过程中psiLC的合成、表达、分子间相互作用和功能。结果表明，虽然psiLC的产生跨越了几个发育阶段，但在正常的B细胞前分化过程中，细胞表面的表达仅限于相对较晚的阶段，在此期间受体交叉连锁不会阻碍细胞生长或B细胞分化。
Surrogate light chain (psiLC) genes are transcriptionally active in progenitor B (pro-B) cells before immunoglobulin genes are rearranged. Current hypothetical models suggest that the psiLC proteins may couple with surrogate or conventional heavy chain proteins to form cell surface receptors that signal the progressive differentiation of pro-B, precursor B (pre-B), and immature B cells. Monoclonal antibodies were produced and used to examine the synthesis, expression, intermolecular interaction, and function of psiLC during B cell differentiation. The results indicate that, while psiLC production spans several developmental stages, cell surface expression is confined to a relatively late stage in normal pre-B cell differentiation, during which receptor cross-linkage does not impede cell growth or B cell differentiation.