STRUCTURE OF A TUMOR-ASSOCIATED ANTIGEN CONTAINING A TANDEMLY REPEATED IMMUNODOMINANT EPITOPE
STRUCTURE OF A TUMOR-ASSOCIATED ANTIGEN CONTAINING A TANDEMLY REPEATED IMMUNODOMINANT EPITOPE
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DOI：
10.1080/07391102.1995.10508837
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发表时间：
1995-10-01
影响因子：
4.4
通讯作者：
GUPTA, G
中科院分区：
文献类型：
--
作者：
FONTENOT, JD;MARIAPPAN, SVS;GUPTA, G
人粘蛋白是T或S糖基化的串联重复蛋白。在乳腺癌中，粘蛋白变成糖基化或未糖基化的二维核磁共振实验是在化学合成的粘蛋白串联重复序列多肽(PDTRPAPGSTAPPAHGVTSA)n上进行的，n=1，3其中(APDTR)构成从乳腺癌患者的肿瘤中分离的抗体的抗原部位。这些研究展示了串联重复序列如何在空间中组装，从而产生整体的三级结构，以及两个相邻重复序列交界处的抗原位点(APDTR)的局部结构和呈现。核磁共振数据显示重复的旋钮状结构由延长的间隔物连接。这些节状突起远离MUC-1的长轴，优势抗原点(APDTR)形成了该节状突起的末端。多个串联重复序列增强了旋钮状结构的刚性和表现性。
Human mucins are T or S glycosylated tandem repeat proteins. In breast cancer, mucins become under or unglycosylated Two-dimensional nuclear magnetic resonance experiments are performed on chemically synthesized mucin tandem repeat polypeptides, (PDTRPAPGSTAPPAHGVTSA)n in the unglycosylated form for n = 1,3 where (APDTR) constitutes the antigenic sites for the antibodies isolated from the tumors in the breast cancer patients. These studies demonstrate how the tandem repeats assemble in space giving rise to the overall tertiary structure, and the local structure and presentation of the antigenic site (APDTR) at the junction of two neighboring repeats. The NMR data reveal repeating knob-like structures connected by extended spacers. The knobs protrude away from the long-axis of Muc-1 and the predominant antigenic site (APDTR) forms the accessible tip of the knob. Multiple tandem repeats enhance the rigidity and presentation of the knob-like structures.