Functional Imaging and Optical Manipulation of Living Cells

Functional Imaging and Optical Manipulation of Living Cells
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发表时间：
1997
期刊：
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影响因子：
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通讯作者：
D. Farkas;B. Tromberg
D. Farkas;B. Tromberg
中科院分区：
其他
文献类型：
--
作者：
D. Farkas;B. Tromberg

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双光子显微镜（TPM）是一种非侵入性生物成像技术，可用于以亚微米分辨率选择性地对高度散射的上皮组织内~ 200 μm深度的细胞活动和光敏剂（PS）定位进行成像。本研究的主要目的是开发一个模型系统，用于了解光动力治疗对生物组织中细胞和细胞外基质重塑的影响。为此目的，已经开发了由胶原、包埋的成纤维细胞和巨噬细胞组成的人工组织模型（RAFT）。TPM用于通过成像胶原/弹性蛋白自体荧光监测PDT后细胞外基质重塑。基质中特定细胞组分对光敏剂的选择性摄取也可以通过TPM可视化。
Two-photon microscopy (TPM) is a non-invasive biological imaging technique that can be used to selectively image cellular activity and photosensitizer (PS) localization within highly scattering epithelial tissues at depths of ∼ 200 μm with submicron resolution. The principal objective of this study was to develop a model system for understanding the impact of photodynamic therapy on cellular and extracellular matrix remodeling in biological tissues. An artificial tissue model (RAFT) composed of collagen, embedded fibroblasts, and macrophage cells has been developed for this purpose. TPM is utilized to monitor extracellular matrix remodeling following PDT by imaging collagen/elastin autofluorescence. Selective uptake of photosensitizers by specific cellular components in the matrix can also be visualized by TPM.