Esterification of an endogenously synthesized lipoxygenase product into granulocyte cellular lipids.
Esterification of an endogenously synthesized lipoxygenase product into granulocyte cellular lipids.
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将内源合成的脂氧合酶产物酯化成粒细胞脂质。
DOI：
10.1021/bi00521a032
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
P. Cuatrecasas
中科院分区：
文献类型：
--
作者：
R. Bonser;M. Siegel;S. Chung;R. McConnell;P. Cuatrecasas
人早幼粒白血病细胞系HL 60经Me 2SO诱导分化为成熟粒细胞。[1- 14 C]与这些细胞孵育过夜的花生四烯酸主要掺入膜磷脂中。刺激这些细胞的钙离子载体，A23187，导致迅速释放酯化花生四烯酸从磷脂酰乙醇胺和磷脂酰胆碱。释放的花生四烯酸通过环氧合酶和脂氧合酶途径代谢成三种主要的羟基化产物，12-L-羟基-5，8，10-十七碳三烯酸（HHT）、5（S）-羟基-6，8，11，14-二十碳四烯酸（5-HETE）和5-（S），12（R）-二羟基-6，8，10，14-二十碳四烯酸（白三烯B）。花生四烯酸也被纳入三酰甘油和磷脂酰肌醇。脂氧合酶产物5-HETE迅速酯化成细胞脂质。离子载体刺激后30分钟，合成的总5-HETE的55%被酯化成磷脂，35%被掺入酰基甘油。相反，花生四烯酸的其他羟基化衍生物（HHT和白三烯B）未掺入酰基甘油或磷脂中。花生四烯酸的羟基化代谢产物酯化成膜磷脂可用于调节许多粒细胞功能。
The human promyelocytic leukemia cell line HL60 can be induced to differentiate into mature granulocytes by exposure to Me2SO. [1-14C]Arachidonic acid incubated overnight with these cells was incorporated mainly into membrane phospholipids. Stimulation of these cells with the calcium ionophore, A23187, resulted in a rapid release of esterified arachidonic acid from phosphatidylethanolamine and phosphatidylcholine. The released arachidonic acid was metabolized via both the cyclooxygenase and lipoxygenase pathways into three major hydroxylated products, 12-L-hydroxy-5,8,10-heptadecatrienoic acid (HHT), 5(S)-hydroxy-6,8,11,14-icosatetraenoic acid (5-HETE), and 5-(S),12(R)-dihydroxy-6,8,10,14-icosatetraenoic acid (leukotriene B). Arachidonic acid was also incorporated into triacylglycerols and phosphatidylinositol. The lipoxygenase product, 5-HETE, was rapidly esterified into cellular lipids. Thirty minutes after ionophore stimulation, 55% of the total 5-HETE synthesized was esterified into phospholipids and 35% incorporated into acylglycerols. In contrast, the other hydroxylated derivatives of arachidonic acid (HHT and leukotriene B) were not incorporated into acylglycerols or phospholipids. Esterification of hydroxylated metabolites of arachidonic acid into membrane phospholipids may serve to regulate a number of granulocyte functions.