Design, construction, and analysis of a novel class of self-folding RNA
Design, construction, and analysis of a novel class of self-folding RNA
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DOI：
10.1016/s0969-2126(02)00739-6
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发表时间：
2002-04-01
期刊：
影响因子：
5.7
通讯作者：
Inoue, T
中科院分区：
文献类型：
--
作者：
Ikawa, Y;Fukada, K;Inoue, T
RNA可以通过其三维结构发挥多种生物学作用。RNA结构生物学的最新进展表明，复杂的RNA三维结构是具有多种三级结构基序的双链螺旋的集合。通过利用RNA的三级结构基序和螺旋，我们设计了一类新的自折叠RNA。在由三个螺旋（P1，P2和P3）组成的RNA中，P1通过四环受体相互作用与P3相互作用，P2形成连续的碱基三联体。制备了这类设计的两种RNA，它们的折叠特性表明它们形成了设计的确定的三级结构。这些RNA可用作构建人工核酶或纳米级材料的模块单元。
RNA can play multiple biological roles through use of its three-dimensional (3-D) structures. Recent advances in RNA structural biology have revealed that complex RNA 3D structures are assemblages of double-stranded helices with a variety of tertiary structural motifs. By employing RNA tertiary structural motifs together with the helices, we designed a novel class of self-folding RNA. In RNA composed of three helices (P1, P2, and P3), P1 interacts with P3 via a tetraloop-receptor interaction and P2 forms consecutive base-triples. Two designed RNAs of this class were prepared and their folding properties indicate that they form defined tertiary structures as designed. These RNAs may be used as modular units for constructing artificial ribozymes or nanometer-scale materials.