Human miR-31 targets radixin and inhibits migration and invasion of glioma cells
Human miR-31 targets radixin and inhibits migration and invasion of glioma cells
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人miR-31靶向根素并抑制神经胶质瘤细胞的迁移和侵袭
DOI：
10.3892/or.2011.1555
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发表时间：
2012-03-01
期刊：
影响因子：
4.2
通讯作者：
Lin, Biaoyang
中科院分区：
文献类型：
--
作者：
Hua, Dasong;Ding, Dong;Lin, Biaoyang
MicroRNAs(MiRNAs)是一类新的短RNA，长度约20-22个核苷酸，通过影响mRNAs的稳定性或翻译，以转录后的方式调节基因的表达，在许多生物过程中发挥重要作用。许多microRNAs与胶质母细胞瘤有关。Mir-31在几种类型的癌症中表达失调，包括结肠癌、乳腺癌、前列腺癌、胃癌和肺癌。然而，miR-31在胶质母细胞瘤中的表达和作用尚不清楚。在本研究中，我们对10例胶质母细胞瘤和7例正常脑组织进行了实时逆转录聚合酶链式反应(RT-PCR)检测。我们发现与正常脑组织相比，miR-31在胶质母细胞瘤中表达下调。异位表达miR-31抑制了U251胶质瘤细胞的迁移和侵袭能力。表达谱分析表明miR-31通过调控迁移和侵袭相关基因影响细胞的迁移和运动过程。最后，我们证明miR-31主要通过抑制蛋白质翻译而不是降解mRNA来靶向Radixin。
MicroRNAs (miRNAs) are a novel group of short RNAs, about 20-22 nucleotide in length, that regulate gene expression in a post-transcriptional manner by affecting the stability or translation of mRNAs and play important roles in many biological processes. Many microRNAs have been implicated in glioblastoma. miR-31 is dysregulated in several types of cancer including colon, breast, prostate, gastric and lung cancers. However, the expression and role of miR-31 in glioblastoma are still unclear. In this study, we performed real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays on 10 glioblastoma and 7 normal brain tissues. We found that miR-31 is down-regulated in glioblastoma compared with normal brain tissues. Ectopic expression of miR-31 inhibited migration and invasion ability of U251 glioma cells. Expression profiling analysis revealed that miR-31 affected the cell migration and motility process by regulating migration and invasion related genes. Finally, we demonstrated that miR-31 targeted radixin predominantly via inhibition of protein translation instead of degradation of mRNA.