THE PETUNIA MADS BOX GENE FBP11 DETERMINES OVULE IDENTITY
THE PETUNIA MADS BOX GENE FBP11 DETERMINES OVULE IDENTITY
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DOI：
10.1105/tpc.7.11.1859
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发表时间：
1995-11-01
期刊：
影响因子：
11.6
通讯作者：
VANTUNEN, AJ
中科院分区：
文献类型：
--
作者：
COLOMBO, L;FRANKEN, J;VANTUNEN, AJ
与调控花分生组织和花器官发育的基因相关的丰富信息相比，关于参与决定和调控胚珠的鉴定和发育的基因的信息有限。我们最近从矮牵牛中分离到花结合蛋白II(FBP11)MADS盒基因，发现它只在胚珠原基中表达，随后在胚珠中表达，表明该基因在胚珠形成中起作用。为了验证这一假说，我们构建了一个重组基因，其中全长的FBP11基因被置于强大的花椰菜花叶病毒35S启动子的控制下。表达该嵌合基因的矮牵牛植株在花冠近轴侧和花瓣远轴侧都有胚珠样结构，详细的形态研究表明这些胚珠状结构是真正的胚珠。通过RNA凝胶印迹分析，研究了异源FBP11基因的表达与紧密相关的FBP7基因和可能的矮牵牛C型同源异型基因FBP6和pMADS3的表达的关系，结果表明FBP11是一个胚珠识别基因，提出了一个新的模型，描述了FBP11作为额外的D类Mads box基因的作用方式。
In contrast to the wealth of information relating to genes regulating floral meristem and floral organ identity, only limited data are available concerning genes that are involved in determining and regulating the identity and development of an ovule, We have recently isolated the floral binding protein II (FBP11) MADS box gene from petunia and found that it is expressed exclusively in ovule primordia and subsequently in the ovules, suggesting a role for this gene in ovule formation, To test this hypothesis, we constructed a recombinant gene in which the full-size FBP11 cDNA was placed under the control of a strong cauliflower mosaic virus 35S promoter. Transgenic petunia plants expressing this chimeric gene have ovulelike structures on the adaxial side of the sepals and the abaxial side of the petals, Detailed morphological studies showed that these ovulelike structures are true ovules, RNA gel blot analysis was performed to investigate ectopic FBP11 expression in relation to the expression of the closely related FBP7 gene and the putative petunia class C-type homeotic genes FBP6 and pMADS3, Our results indicate that FBP11 represents an ovule identity gene, A new model describing the mode of action of FBP11 as an additional class D MADS box gene is presented.