Human mesenchymal stem cells promote growth of osteosarcoma: Involvement of interleukin-6 in the interaction between human mesenchymal stem cells and Saos-2
Human mesenchymal stem cells promote growth of osteosarcoma: Involvement of interleukin-6 in the interaction between human mesenchymal stem cells and Saos-2
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DOI：
10.1111/j.1349-7006.2010.01731.x
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发表时间：
2010-12-01
期刊：
影响因子：
5.7
通讯作者：
Tang, Ting-Ting
中科院分区：
文献类型：
--
作者：
Bian, Zhen-Yu;Fan, Qi-Ming;Tang, Ting-Ting
我们先前的研究表明，外源性人间充质干细胞(HMSCs)靶向已建立的骨肉瘤，并促进其在体内的生长和肺转移。作为后续研究，本研究旨在探讨hMSCs如何通过自分泌/旁分泌通讯与Saos-2相互作用。结果表明，hMSCs与Saos-2共注射于裸鼠胫骨近端可促进肿瘤生长和进展。体外条件培养液对Saos-2和hMSCs的增殖均有促进作用，其中IL-6起重要作用。SAOS-2条件培养液可抑制hMSCs的成骨分化，IL-6也参与其中。此外，观察到hMSCs在成骨过程中IL-6的分泌减少，而在Saos-2的条件培养液中IL-6的分泌增加。根据这些数据，我们认为在hMSCs与Saos-2的相互作用中存在着IL-6的正反馈回路。(《癌症科学》2010；101：2554-2560)。
Our previous study showed that exogenous human mesenchymal stem cells (hMSCs) targeted established osteosarcoma and promoted its growth and pulmonary metastasis in vivo. As a follow-up, the present study aimed to investigate how hMSCs would interact with Saos-2 through autocrine/paracrine communication. The results showed that co-injection of hMSCs with Saos-2 into the proximal tibia of nude mice could promote tumor growth and progression. In vitro, the proliferation of Saos-2 and hMSCs was promoted by each other's conditioned medium, in which interleukin-6 (IL-6) played an important role. Osteogenic differentiation of hMSCs could be inhibited by conditioned medium of Saos-2, in which IL-6 was also involved. Furthermore, decreased IL-6 secretion by hMSCs during its osteogenesis and increased IL-6 secretion in response to conditioned medium of Saos-2 were observed. Based on these data, we suggest that there was a positive feedback loop of IL-6 in the interaction between hMSCs and Saos-2. (Cancer Sci 2010; 101: 2554-2560).