Biologically important nucloesides: modern trends in biotechnology and application
Biologically important nucloesides: modern trends in biotechnology and application
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DOI：
10.1016/j.mencom.2011.03.001
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发表时间：
2011-03-01
影响因子：
1.9
通讯作者：
Miroshnikov, Anatoly I.
中科院分区：
文献类型：
--
作者：
Mikhailopulo, Igor A.;Miroshnikov, Anatoly I.
概述了具有生物化学和医学重要性的核苷生物技术的现代趋势。重点是在一般的化学-酶法合成核苷的酶转化。（i）感兴趣的核酸代谢酶作为生物催化剂的功能，（ii）使用糖修饰的核苷作为碳水化合物残基的供体和杂环碱基作为受体由核苷磷酸化酶和N-脱氧核糖基转移酶催化的转糖基化反应，（iii）使用D-戊呋喃糖5-磷酸或α-D-戊呋喃糖1-磷酸作为通用糖基化底物的杂项转化，（iv）逆合成，（v）D-戊糖一锅法转化为核苷。
Modern trends in biotechnology of nucleosides of biochemical and medicinal importance are outlined. Emphasis is made on the enzymatic transformations within the general chemo-enzymatic approach to the synthesis of nucleosides. Some aspects of (i) the functioning of enzymes of nucleic acid metabolism of interest as biocatalysts, (ii) the transglycosylation reaction using sugar modified nucleosides as donors of carbohydrate residues and heterocyclic bases as acceptors catalyzed by nucleoside phosphorylases and N-deoxyribosyltransferases, (iii) the miscellaneous transformations employing D-pentofuranose 5-phosphates or alpha-D-pentofuranose 1-phosphates as universal glycosylatig substrates, (iv) the retrosynthesis, and (v) the one-pot transformation of D-pentoses into nucleosides are considered.