Circulating factors cause proteinuria in parabiotic zebrafish.
Circulating factors cause proteinuria in parabiotic zebrafish.
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DOI：
10.1016/j.kint.2019.02.013
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发表时间：
2019-08
影响因子：
19.6
通讯作者：
J. Müller-Deile;H. Schenk;P. Schroder;K. Schulze;P. Bolaños-Palmieri;F. Siegerist;N. Endlich;H. Haller;M. Schiffer
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文献类型：
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作者：
J. Müller-Deile;H. Schenk;P. Schroder;K. Schulze;P. Bolaños-Palmieri;F. Siegerist;N. Endlich;H. Haller;M. Schiffer
蛋白尿可由肾小球滤过屏障（GFB）的任何组成部分的损伤引起。为了确定循环通透性因子在肾小球损伤中的作用，我们开发了一个基于异种共生的斑马鱼模型，以在斑马鱼幼虫之间产生共同循环。在一条斑马鱼幼体中诱导了一种morpholino介导的足细胞特异性基因（nephronectin）的敲低，然后将其融合到一条未处理的鱼中。值得注意的是，在被操纵的鱼和异种融合的伴侣中存在蛋白尿和肾小球损伤。因此，循环渗透性因子可能由蛋白尿诱导，即使诱导足细胞基因失调是初始原因。
Proteinuria can be induced by impairment of any component of the glomerular filtration barrier (GFB). To determine the role of circulating permeability factors on glomerular damage, we developed a parabiosis-based zebrafish model to generate a common circulation between zebrafish larvae. A morpholino-mediated knockdown of a podocyte specific gene (nephronectin) was induced in one zebrafish larva which was then fused to an un-manipulated fish. Notably, proteinuria and glomerular damage were present in the manipulated fish and in the parabiotically-fused partner. Thus, circulating permeability factors may be induced by proteinuria even when an induced podocyte gene dysregulation is the initiating cause.