Common developmental pathway for primitive erythrocytes and multipotent hematopoietic progenitors in early mouse development.
Common developmental pathway for primitive erythrocytes and multipotent hematopoietic progenitors in early mouse development.
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DOI：
10.1016/j.stemcr.2013.10.008
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发表时间：
2013
影响因子：
5.9
通讯作者：
Yamazaki H
中科院分区：
文献类型：
--
作者：
Yamane T;Washino A;Yamazaki H
造血系统的发育以多步骤的方式进行。原始红细胞是第一个被观察到的造血细胞，它是在发育中的胚胎中瞬时产生的。多系淋巴造血发生在原始红细胞生成之后。然而，组成胚胎造血系统的细胞的谱系关系尚未得到很好的表征。为了阐明这个过程，有必要仔细分析分化成这些细胞谱系的胚胎细胞。我们在小鼠胚胎干细胞培养物和小鼠胚胎中鉴定了原始红细胞和多能造血细胞的共同前体。定义为 CD45−CD41+AA4.1− 细胞的子集显示出在单细胞水平上产生原始红细胞和淋巴细胞的双潜能能力。在造血干细胞 (HSC) 出现之前，细胞群就已存在于体内。我们的研究结果表明，原始红细胞和淋巴骨髓细胞并不是完全独立的细胞谱系，这些前体细胞在HSC出现之前构成了胚胎造血系统。原始红细胞和淋巴骨髓祖细胞具有双能前体细胞 前体细胞以不同的波形成原始红细胞和淋巴骨髓细胞 共同的前体细胞在卵黄囊和胚胎本身中被发现 原始红细胞和淋巴细胞可以源自单个前体细胞 Yamane 和同事表明存在双能细胞 胚胎中短暂产生的原始红细胞和在小鼠发育早期阶段提供淋巴和骨髓细胞的多能造血祖细胞的阶段。原始红细胞和淋巴细胞不是完全独立的细胞谱系。
Development of the hematopoietic system proceeds in a multistep manner. Primitive erythrocytes are the first hematopoietic cells to be observed that were produced transiently in developing embryos. Multilineage lymphohematopoiesis occurs after the primitive erythropoiesis. However, the lineage relationship of cells that comprise embryonic hematopoietic system is not well characterized. To clarify this process, careful analyses of the embryonic cells that differentiate into these cell lineages are necessary. We identified the common precursors of primitive erythrocytes and multipotent hematopoietic cells in mouse embryonic stem cell cultures and mouse embryos. A subset defined as CD45−CD41+AA4.1− cells showed bipotential capability to produce primitive erythrocytes and lymphomyeloid cells at the single-cell level. The cell population was present in vivo before hematopoietic stem cells (HSCs) appeared. Our results show that primitive erythrocytes and lymphomyeloid cells are not completely separate cell lineages, and these precursors comprise the embryonic hematopoietic system before HSC emergence. Primitive erythrocytes and lymphomyeloid progenitors have bipotent precursors The precursors form primitive erythroid and lymphomyeloid cells in distinct waves The common precursors are noted in the yolk sac and embryo proper Primitive erythrocytes and lymphocytes can be derived from a single precursor cell Yamane and colleagues show that there is a bipotential cell stage for the primitive erythrocytes that are produced transiently in embryos and multipotent hematopoietic progenitors that supply lymphoid and myeloid cells during the early stage of mouse development. Primitive erythrocytes and lymphomyeloid cells are not completely separate cell lineages.