Comparative Proteomics of Salt-Tolerant and Salt-Sensitive Maize Inbred Lines to Reveal the Molecular Mechanism of Salt Tolerance
Comparative Proteomics of Salt-Tolerant and Salt-Sensitive Maize Inbred Lines to Reveal the Molecular Mechanism of Salt Tolerance
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DOI：
10.3390/ijms20194725
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Ren, Bin
中科院分区：
文献类型：
--
作者：
Chen, Fenqi;Fang, Peng;Ren, Bin
盐胁迫是导致玉米产量和品质严重下降的主要非生物胁迫之一。因此，揭示玉米耐盐性的分子机制非常重要。为了了解玉米耐盐性的分子机制，本研究使用了耐盐玉米自交系8723和盐敏感玉米自交系P138。采用iTRAQ方法对180 mM盐胁迫10 d下2个玉米自交系幼苗根系进行比较蛋白质组学研究。共鉴定出1056个差异表达蛋白（DEPs）。在盐胁迫下，8723共检测到626个DEPs，其中378个表达上调，248个表达下调。在P138中检测到473个DEPs，其中212个上调，261个下调。文氏图分析表明，2个自交系中有17个DEPs表达上调，12个DEPs表达下调。另外，8个DEPs在细胞系8723中表达上调，而在P138中表达下调，6个DEPs在细胞系8723中表达下调，而在P138中表达上调。在盐胁迫8723中，DEPs主要与苯丙烷类化合物的生物合成、淀粉和蔗糖代谢以及丝裂原活化蛋白激酶（MAPK）信号通路有关。有趣的是，DEP仅与P138中的氮代谢途径相关。与P138相比，8723根系对盐胁迫的响应具有更强的保水能力、渗透调节能力、抗氧化酶协同作用、能量供应能力、信号转导能力、氨解毒能力、脂质代谢能力和核酸合成能力。基于蛋白质组测序信息，通过实时定量PCR（qRT-PCR）分析8种DEPs的丰度变化与相应mRNA水平的关系。本研究的结果可能会阐明玉米耐盐机制和耐盐育种的一些细节。
Salt stress is one of the key abiotic stresses that causes great loss of yield and serious decrease in quality in maize (Zea mays L.). Therefore, it is very important to reveal the molecular mechanism of salt tolerance in maize. To acknowledge the molecular mechanisms underlying maize salt tolerance, two maize inbred lines, including salt-tolerant 8723 and salt-sensitive P138, were used in this study. Comparative proteomics of seedling roots from two maize inbred lines under 180 mM salt stress for 10 days were performed by the isobaric tags for relative and absolute quantitation (iTRAQ) approach. A total of 1056 differentially expressed proteins (DEPs) were identified. In total, 626 DEPs were identified in line 8723 under salt stress, among them, 378 up-regulated and 248 down-regulated. There were 473 DEPs identified in P138, of which 212 were up-regulated and 261 were down-regulated. Venn diagram analysis showed that 17 DEPs were up-regulated and 12 DEPs were down-regulated in the two inbred lines. In addition, 8 DEPs were up-regulated in line 8723 but down-regulated in P138, 6 DEPs were down-regulated in line 8723 but up-regulated in P138. In salt-stressed 8723, the DEPs were primarily associated with phenylpropanoid biosynthesis, starch and sucrose metabolism, and the mitogen-activated protein kinase (MAPK) signaling pathway. Intriguingly, the DEPs were only associated with the nitrogen metabolism pathway in P138. Compared to P138, the root response to salt stress in 8723 could maintain stronger water retention capacity, osmotic regulation ability, synergistic effects of antioxidant enzymes, energy supply capacity, signal transduction, ammonia detoxification ability, lipid metabolism, and nucleic acid synthesis. Based on the proteome sequencing information, changes of 8 DEPs abundance were related to the corresponding mRNA levels by quantitative real-time PCR (qRT-PCR). Our results from this study may elucidate some details of salt tolerance mechanisms and salt tolerance breeding of maize.