Immunogenicity and protective efficacy of recombinant Campylobacter jejuni flagellum-secreted proteins in mice
Immunogenicity and protective efficacy of recombinant Campylobacter jejuni flagellum-secreted proteins in mice
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DOI：
10.1128/iai.00076-08
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发表时间：
2008-07-01
影响因子：
3.1
通讯作者：
Guerry, Patricia
中科院分区：
文献类型：
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作者：
Baqar, Shahida;Applebee, Lisa A.;Guerry, Patricia
通过小鼠模型比较了空肠弯曲菌鞭毛分泌的三种蛋白FlaC、FspA 1和FspA 2的免疫原性和保护效力。在有或没有LTR 192 G作为佐剂的情况下，用每种蛋白质鼻内免疫小鼠，并用空肠弯曲杆菌81-176或CG 8486鼻内攻击。所有三种蛋白质都具有免疫原性，尽管FspA 1诱导了最高水平的血清免疫球蛋白G（IgG）和粪便伊加。尽管具有免疫原性，但FlaC仅提供18%的针对来自空肠弯曲杆菌的疾病的保护81-176。用FspA 1免疫导致57.8%的无佐剂保护或63.8%的有佐剂保护对抗81-176的同源攻击。或者，用FspA 2免疫提供了38.4%（无佐剂）或47.2%（有佐剂）的针对来自用CG 8486同源攻击的疾病的保护。与FspA 2相反，当与（31.2%）或不与（44.8%）LTR 192 G一起递送时，FspA 1提供针对空肠弯曲菌CG 8486的一些异源保护。这些结果表明，FspA 1可能是一个很好的亚单位疫苗候选人针对空肠弯曲菌病。
Immunogenicity and protective efficacy of three Campylobacter jejuni flagellum-secreted proteins, FlaC, FspA1, and FspA2, were compared by use of a mouse model. Mice were immunized intranasally with each protein with or without LTR192G as the adjuvant and challenged intranasally with C.jejuni 81-176 or CG8486. All three proteins were immunogenic, although FspA1 induced the highest levels of serum immunoglobulin G (IgG) and fecal IgA. Although immunogenic, FlaC provided only 18% protection against disease from C.jejuni 81-176. Immunization with FspA1 resulted in 57.8% protection without adjuvant or 63.8% protection with adjuvant against homologous challenge with 81-176. Alternatively, immunization with FspA2 provided 38.4% (without adjuvant) or 47.2% (with adjuvant) protection against disease from homologous challenge with CG8486. In contrast to FspA2, FspA1 provided some heterologous protection against C.jejuni CG8486 when delivered with (31.2%) or without (44.8%) LTR192G. These results suggest that FspA1 may be a good subunit vaccine candidate against C.jejuni disease.