Localization of Eutr2, a locus controlling susceptibility to DES-induced uterine inflammation and pyometritis, to RNO5 using a congenic rat strain.
Localization of Eutr2, a locus controlling susceptibility to DES-induced uterine inflammation and pyometritis, to RNO5 using a congenic rat strain.
复制标题
使用同类大鼠品系对 Eutr2（控制 DES 诱导的子宫炎症和子宫脓炎易感性的基因座）进行 RNO5 定位。
DOI：
10.1007/s00335-005-0071-6
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Wendell,DouglasL
中科院分区：
文献类型：
--
作者：
Pandey,Jyotsna;Gould,KarenA;McComb,RodneyD;Shull,JamesD;Wendell,DouglasL
在一些大鼠品系中，长期给药外源性雌激素可诱发脓子宫炎，这是一种与感染相关的子宫炎症，这表明对雌激素诱导的炎症和脓子宫炎的易感性存在遗传变异。在这篇文章中，我们报道了在使用合成雌激素己烯雌酚（DES）治疗10周后，Fisher 344 （F344）大鼠表现出适度的子宫炎症和0%的脓腔炎发生率。相比之下，在相同的实验条件下，褐挪威（BN）大鼠表现出明显的炎症和100%的脓脓炎发生率。同样地，我们也观察到，在一个同源大鼠品系中，来自BN品系的RNO5片段携带在F344品系中，子宫炎症和脓腔炎的发生率为100%。这些数据表明，RNO5上的一个位点既控制des诱导的子宫炎症的程度，也控制des诱导的脓脓炎的易感性。这个位点被命名为deutr2，与eutr1位点位于RNO5的同一片段中，这使得BN与8月×哥本哈根9935爱尔兰（ACI）菌株杂交产生的F2群体对e2诱导的脓腔炎具有易感性。
In some rat strains chronic administration of exogenous estrogens induces pyometritis, an inflammation of the uterus associated with infection, suggesting that there is genetic variation in susceptibility to estrogen-induced inflammation and pyometritis. In this article we report that following 10 weeks of treatment with the synthetic estrogen diethylstilbestrol (DES), Fisher 344 (F344) rats exhibit modest uterine inflammation and a 0% incidence of pyometritis. By contrast, under identical experimental conditions, Brown Norway (BN) rats exhibit significant inflammation and a 100% incidence of pyometritis. Similarly, we also observed profound uterine inflammation and a 100% incidence of pyometritis in a congenic rat strain in which a segment of RNO5 from the BN strain is carried on the F344 strain. These data suggest that a locus on RNO5 controls both the magnitude of DES-induced uterine inflammation and susceptibility to DES-induced pyometritis. This locus, designatedEutr2, resides within the same segment of RNO5 as theEutr1locus, which confers susceptibility to E2-induced pyometritis in an F2 population generated in a cross between the BN and August × Copenhagen 9935, Irish (ACI) strains.