Degradation of connexins from the plasma membrane is regulated by inhibitors of protein synthesis.
Degradation of connexins from the plasma membrane is regulated by inhibitors of protein synthesis.
复制标题
连接蛋白从质膜的降解受到蛋白质合成抑制剂的调节。
DOI：
10.1080/cac.10.4-6.329.333
复制
发表时间：
2003
影响因子：
--
通讯作者：
Musil,LindaS
中科院分区：
文献类型：
--
作者：
VanSlyke,JudyK;Musil,LindaS
关于质膜连接蛋白周转的机制和调控知之甚少。我们已经使用了细胞表面生物素化，免疫荧光显微镜，和刮负载染料转移试验相结合，研究蛋白质合成抑制剂放线菌酮对连接蛋白43和连接蛋白32后，其运输到质膜的影响。所获得的结果表明，放线菌酮抑制营业额的间隙连接组装缺陷和高效细胞的表面连接蛋白。此外，从放线菌酮破坏中保存的细胞表面连接蛋白可以组装成寿命长的功能性间隙连接斑块。这些发现支持这样的概念，即下调连接蛋白降解的质膜可以作为一种机制，以增强间隙连接介导的细胞间通讯。
Little is known about the mechanism and regulation of connexin turnover from the plasma membrane. We have used a combination of cell surface biotinylation, immunofluorescence microscopy, and scrape-load dye transfer assays to investigate the effect of the protein synthesis inhibitor cycloheximide on connexin43 and connexin32 after their transport to the plasmalemma. The results obtained demonstrate that cycloheximide inhibits the turnover of connexins from the surface of both gap junction assembly-deficient and -efficient cells. Moreover, cell surface connexin saved from destruction by cycloheximide can assemble into long-lived, functional gap junctional plaques. These findings support the concept that downregulation of connexin degradation from the plasma membrane can serve as a mechanism to enhance gap junction-mediated intercellular communication.