Characterization of the cassette containing genes for type 3 capsular polysaccharide biosynthesis in Streptococcus pneumoniae.
Characterization of the cassette containing genes for type 3 capsular polysaccharide biosynthesis in Streptococcus pneumoniae.
复制标题
DOI：
10.1084/jem.181.3.973
复制
发表时间：
1995-03-01
期刊：
影响因子：
--
通讯作者：
Yother J
中科院分区：
文献类型：
--
作者：
Dillard JP;Vandersea MW;Yother J
荚膜多糖是肺炎链球菌的主要毒力因子。在此之前，我们从S. pneumoniae染色体特异性地产生3型荚膜多糖。现在，通过对该区域进行测序，并在基因上和体外胶囊合成测定中表征突变，我们已经将推定的功能分配给了类型特异性基因的产物。在作图研究中使用该区域右端的DNA，我们获得了进一步的证据，表明每个血清型的荚膜基因包含在位于该区域附近的基因盒中。我们已经从3型染色体上克隆了盒左端侧翼的区域，并发现它在S。肺炎染色体。DNA序列和杂交数据提出了一种胶囊基因盒重组的模型，不仅描述了胶囊基因的替换，而且还提出了对二元胶囊类型形成和新胶囊类型产生的解释。
The capsular polysaccharide is the major virulence factor of Streptococcus pneumoniae. Previously, we identified and cloned a region from the S. pneumoniae chromosome specific for the production of type 3 capsular polysaccharide. Now, by sequencing the region and characterizing mutations genetically and in an in vitro capsule synthesis assay, we have assigned putative functions to the products of the type-specific genes. Using DNA from the right end of the region in mapping studies, we have obtained further evidence indicating that the capsule genes of each serotype are contained in a gene cassette located adjacent to this region. We have cloned the region flanking the left end of the cassette from the type 3 chromosome and have found that it is repeated in the S. pneumoniae chromosome. The DNA sequence and hybridization data suggest a model for recombination of the capsule gene cassettes that not only describes the replacement of capsule genes, but also suggests an explanation for binary capsule type formation, and the creation of novel capsule types.