Biodegradable nano-films for capture and non-invasive release of circulating tumor cells.
Biodegradable nano-films for capture and non-invasive release of circulating tumor cells.
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DOI：
10.1016/j.biomaterials.2015.06.036
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发表时间：
2015-10
期刊：
影响因子：
14
通讯作者：
Hammond PT
中科院分区：
文献类型：
--
作者：
Li W;Reátegui E;Park MH;Castleberry S;Deng JZ;Hsu B;Mayner S;Jensen AE;Sequist LV;Maheswaran S;Haber DA;Toner M;Stott SL;Hammond PT
从全血中选择性分离和纯化循环肿瘤细胞（CTCs）对临床医学和生物学研究都具有重要意义。目前用于执行该任务的技术使分离出的细胞承受过度压力，这降低了细胞活力，并可能诱导表型改变，从而丢失了有关分离细胞的有价值信息。我们在微流控芯片表面展示了一种可生物降解的纳米薄膜涂层，它可用于有效捕获以及无创释放癌细胞系，如PC - 3、LNCaP、DU 145、H1650和H1975。我们应用层层自组装（LbL）技术，通过互补相互作用使用多种材料创建了一个超薄涂层库。通过开发一种具有基于亲和力的细胞捕获表面的LbL纳米薄膜涂层，它能够从全血中选择性地分离癌细胞，并且可以根据指令快速降解，我们能够温和地分离癌细胞并在不损害细胞活力或增殖潜力的情况下回收它们。我们的方法有能力克服实际障碍，并为下游分析提供有活力的癌细胞，例如活细胞成像、单细胞基因组学以及回收细胞的体外细胞培养。此外，使用LbL修饰的微芯片也能够捕获、识别癌症患者的循环肿瘤细胞，并成功将其释放。
Selective isolation and purification of circulating tumor cells (CTCs) from whole blood is an important capability for both clinical medicine and biological research. Current techniques to perform this task place the isolated cells under excessive stresses that reduce cell viability, and potentially induce phenotype change, therefore losing valuable information about the isolated cells. We present a biodegradable nano-film coating on the surface of a microfluidic chip, which can be used to effectively capture as well as non-invasively release cancer cell lines such as PC-3, LNCaP, DU 145, H1650 and H1975. We have applied layer-by-layer (LbL) assembly to create a library of ultrathin coatings using a broad range of materials through complementary interactions. By developing an LbL nano-film coating with an affinity-based cell-capture surface that is capable of selectively isolating cancer cells from whole blood, and that can be rapidly degraded on command, we are able to gently isolate cancer cells and recover them without compromising cell viability or proliferative potential. Our approach has the capability to overcome practical hurdles and provide viable cancer cells for downstream analyses, such as live cell imaging, single cell genomics, and in vitro cell culture of recovered cells. Furthermore, CTCs from cancer patients were also captured, identified, and successfully released using the LbL-modified microchips.