DIFFERENTIAL PRODUCTION OF INTERFERON-GAMMA AND INTERLEUKIN-4 IN RESPONSE TO TH1-STIMULATING AND TH2-STIMULATING PATHOGENS BY GAMMA-DELTA T-CELLS IN-VIVO
DIFFERENTIAL PRODUCTION OF INTERFERON-GAMMA AND INTERLEUKIN-4 IN RESPONSE TO TH1-STIMULATING AND TH2-STIMULATING PATHOGENS BY GAMMA-DELTA T-CELLS IN-VIVO
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DOI：
10.1038/373255a0
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发表时间：
1995-01-19
期刊：
影响因子：
64.8
通讯作者：
LEPPER, H
中科院分区：
文献类型：
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作者：
FERRICK, DA;SCHRENZEL, MD;LEPPER, H
暴露于各种病原体可刺激CD 4阳性T细胞产生至少两种细胞因子模式（1-4）导致γ-干扰素分泌的应答（IFN-γ）、α-干扰素和白细胞介素-2（IL-2）被归类为辅助性T细胞-1（Th 1）（5，6）; CD 4（+）T细胞产生IL-4、IL-5、IL-9、IL-10和IL-13称为辅助性T细胞2应答（Th 2）（5，6）。CD 4（+）T细胞分化为Th 1或Th 2细胞受到细胞因子环境的影响，在该环境中发生初始抗原引发（7-9）。在这里，我们使用流式细胞术，以确定细胞内细胞因子的存在（cytoflow），并分析T细胞生产的IFN-γ和IL-4感染单核细胞增生李斯特菌或日本圆线虫巴西的小鼠。我们发现，携带ya受体的T细胞通过产生与适当的辅助性T细胞反应相关的细胞因子来区分这两种病原体的感染早期。我们的研究结果表明，γ δ T细胞参与建立初级免疫应答。
EXPOSURE to various pathogens can stimulate at least two patterns of cytokine production by CD4-positive T cells(1-4) Responses that result in secretion of interferon-gamma (IFN-gamma), lymphotoxin and interleukin-2 (IL-2) are classified as T-helper-1 (Th1)(5,6); CD4(+) T-cell production of IL-4, IL-5, IL-9, IL-10 and IL-13 is called a T-helper-2 response (Th2)(5,6). Differentiation of CD4(+) T cells into either Th1 or Th2 cells is influenced by the cytokine milieu in which the initial antigen priming occurs(7-9). Here we use flow cytometry to identify the presence of intracellular cytokines (cytoflow) and analyse T-cell production of IFN-gamma and IL-4 from mice infected with Listeria monocytogenes or Nippostrongylus brasiliensis. We show that T cells bearing ya receptors discriminate early in infection between these two pathogens by producing cytokines associated with the appropriate T-helper response. Our results demonstrate that gamma delta T cells are involved in establishing primary immune responses.