Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM
Enhanced 2,3-butanediol production by Klebsiella pneumoniae SDM
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肺炎克雷伯菌 SDM 提高 2,3-丁二醇产量
DOI：
10.1007/s00253-008-1732-7
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发表时间：
2009-02-01
影响因子：
5
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Ma, Cuiqing;Wang, Ailong;Xu, Ping
通过肺炎克雷伯氏菌SDM进行增强的2，3-丁二醇（BD）生产。研究了K. pneumoniae SDM在摇瓶发酵中进行了统计学优化。通过Plackett-Burman二水平设计确定玉米浆粉和（NH（4））（2）HPO（4）为最显著因素。采用最陡爬坡试验法对两因素的最优区域进行了拟合，并采用中心复合设计确定了两因素的最优水平。采用优化的培养基进行补料分批发酵。肺炎SDM。BD生产，然后在5升生物反应器中进行了研究，采用不同的补料分批策略，包括脉冲补料分批，恒定的补料速率补料分批，恒定的残留葡萄糖浓度补料分批，和指数补料分批。通过恒定残余葡萄糖浓度的进料策略，在38 h时获得最大BD浓度150 g/l，二醇生产率为4.21 g/l h。据我们所知，这些结果是BD发酵的新记录。
Enhanced 2,3-butanediol (BD) production was carried out by Klebsiella pneumoniae SDM. The nutritional requirements for BD production by K. pneumoniae SDM were optimized statistically in shake flask fermentations. Corn steep liquor powder and (NH(4))(2)HPO(4) were identified as the most significant factors by the two-level Plackett-Burman design. Steepest ascent experiments were applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal levels. The optimal medium was used to perform fed-batch fermentations with K. pneumoniae SDM. BD production was then studied in a 5-l bioreactor applying different fed-batch strategies, including pulse fed batch, constant feed rate fed batch, constant residual glucose concentration fed batch, and exponential fed batch. The maximum BD concentration of 150 g/l at 38 h with a diol productivity of 4.21 g/l h was obtained by the constant residual glucose concentration feeding strategy. To the best of our knowledge, these results were new records on BD fermentation.