Strontium modulates osteogenic activity of bone cement composed of bioactive borosilicate glass particles by activating Wnt/β-catenin signaling pathway
Strontium modulates osteogenic activity of bone cement composed of bioactive borosilicate glass particles by activating Wnt/β-catenin signaling pathway
复制标题
锶通过激活Wnt/β-catenin信号通路调节由生物活性硼硅酸盐玻璃颗粒组成的骨水泥的成骨活性
DOI：
10.1016/j.bioactmat.2020.02.016
复制
发表时间：
2020-06-01
影响因子：
18.9
通讯作者：
Pan, Haobo
中科院分区：
文献类型：
--
作者：
Cui, Xu;Zhang, Yadong;Pan, Haobo
需要使用微创手术的合成移植物来重建大型骨缺损区。我们先前的研究表明，在生物活性硼酸盐玻璃水泥中掺入锶可以提高体内的成骨能力。然而，骨水泥中锶的含量能提供最佳的物理化学性质和刺激骨再生的能力的组合，以及这种刺激的潜在分子机制仍有待确定。在本研究中，用不同量的锶(0~12摩尔%的锶)取代生物活性的硼硅酸盐玻璃颗粒，制成骨水泥，并在体外和体内进行评价。随着玻璃中锶含量的增加，水泥的凝结时间延长。在PBS中浸泡时，随着锶取代量的增加，水泥降解并转化为羟基磷灰石(HA)的速度变慢。体外释放的Sr2+对人骨髓间充质干细胞(HBMSCs)的增殖、分化和矿化有调节作用。用6mol%SRO替代颗粒组成的骨水泥(BG6sr)能最大限度地增强成骨特性。与0mol%和9mol%SRO替代骨水泥相比，BG6sr骨水泥植入兔股骨髁缺损处时，能更好地支持种植体周围骨形成和骨-种植体接触。其机制可能与激活Wnt/β-catenin信号通路参与hBMSCs的成骨分化有关。这些结果表明，BG6sr骨水泥具有良好的物理化学性质和生物学性能，可用于骨缺损的微创修复。
There is a need for synthetic grafts to reconstruct large bone defects using minimal invasive surgery. Our previous study showed that incorporation of Sr into bioactive borate glass cement enhanced the osteogenic capacity in vivo. However, the amount of Sr in the cement to provide an optimal combination of physicochemical properties and capacity to stimulate bone regeneration and the underlying molecular mechanism of this stimulation is yet to be determined. In this study, bone cements composed of bioactive borosilicate glass particles substituted with varying amounts of Sr (0 mol% to 12 mol% SrO) were created and evaluated in vitro and in vivo. The setting time of the cement increased with Sr substitution of the glass. Upon immersion in PBS, the cement degraded and converted more slowly to HA (hydroxyapatite) with increasing Sr substitution. The released Sr2+ modulated the proliferation, differentiation, and mineralization of hBMSCs (human bone marrow mesenchymal stem cells) in vitro. Osteogenic characteristics were optimally enhanced with cement (designated BG6Sr) composed of particles substituted with 6mol% SrO. When implanted in rabbit femoral condyle defects, BG6Sr cement supported better peri-implant bone formation and bone-implant contact, comparing to cements substituted with 0mol% or 9mol% SrO. The underlying mechanism is involved in the activation of Wnt/beta-catenin signaling pathway in osteogenic differentiation of hBMSCs. These results indicate that BG6Sr cement has a promising combination of physicochemical properties and biological performance for minimally invasive healing of bone defects.yy