Effect of osmolalities comparable to those of the renal medulla on function of human polymorphonuclear leukocytes.
Effect of osmolalities comparable to those of the renal medulla on function of human polymorphonuclear leukocytes.
复制标题
与肾髓质渗透压相当的渗透压对人多形核白细胞功能的影响。
DOI：
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发表时间：
1972
影响因子：
6.4
通讯作者：
Z. A. McGee
中科院分区：
文献类型：
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作者：
R. Bryant;M. Sutcliffe;Z. A. McGee
研究了高渗透压对人多形核白细胞 (PMN) 迁移、聚集、粘附、吞噬作用和细胞内细菌杀灭的影响，以确定与肾髓质中存在的高渗透压相比是否直接抑制这些功能或仅作为补体抑制的结果。高渗氯化钠或蔗糖似乎可以直接抑制中性粒细胞的迁移、聚集和粘附，因为两者在不使补体失活的渗透压下抑制这些功能。此外，发现通过添加高渗蔗糖消除了通过螯合剂在无补体活性的血浆中维持的白细胞功能。高渗氯化钠、尿素或蔗糖会损害细胞内细菌的杀灭作用。白细胞的某些功能在渗透压为300-400毫渗摩尔/千克时增强。 PMN 的聚集、粘附和迁移对渗透压抑制比吞噬作用更敏感，这表明肾髓质对感染的敏感性增加可能主要是由于白细胞输送到感染部位的渗透压抑制所致。
The effect of hyperosmolality on human polymorphonuclear-leukocyte (PMN) migration, aggregation, adhesion, phagocytosis, and intracellular killing of bacteria was studied to determine whether hyperosmolality comparable to that present in the renal medulla suppressed these functions directly or only as a result of inhibition of complement. Hypertonic sodium chloride or sucrose appeared to suppress migration, aggregation, and adhesion of PMNs directly, because both inhibited these functions at osmolalities that did not inactivate complement. Furthermore, it was found that leukocyte functions that were maintained in plasma freed of complement activity by chelating agents were abolished by the addition of hypertonic sucrose. Intracellular killing of bacteria was impaired by hypertonic sodium chloride, urea, or sucrose. Certain functions of leukocytes were enhanced at osmolalities of 300-400 milliosmols/kg. Aggregation, adhesion, and migration of PMNs were more sensitive to osmotic suppression than was phagocytosis, suggesting that increased susceptibility of the renal medulla to infection might result primarily from osmotic inhibition of leukocyte delivery to the site of infection.