Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9.
Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9.
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DOI：
10.1016/j.ecoenv.2021.112789
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发表时间：
2021-09
影响因子：
6.8
通讯作者：
Jing Liu;Aijia Zhang;Y. Liu;Zhe Liu;Yu Liu;Xijun Wu
中科院分区：
文献类型：
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作者：
Jing Liu;Aijia Zhang;Y. Liu;Zhe Liu;Yu Liu;Xijun Wu
降解速度慢、转化效率低是多环芳烃（PAHs）生物降解的主要问题。本研究选择芘作为目标PAH，研究亚铁离子和三价铁离子对芘降解的影响。研究了红球菌红球菌株L9生物降解过程中的驱动效应和机制，包括芘和铁离子之间的相互作用以及细菌的生理反应。结果表明，铁离子不会促进细菌生长，但会提高细菌的芘去除能力，从而提高芘生物降解的总效率。该过程始于Fe(III)和芘之间初步形成“阳离子-π”，随后驱动芘去除过程并加速细菌代谢过程。此外，粗酶溶液中蛋白质浓度、儿茶酚双加氧酶（C12O和C23O）活性以及细胞内蛋白质调节的显着增加表明细菌在铁离子增强的芘降解过程中出现积极反应。
A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process byRhodococcus ruberstrain L9, were investigated. The results showed that iron ions did not enhance bacterial growth but improved bacteria’s pyrene removal capacity, contributing to the total efficiency of pyrene biodegradation. The process started with an initial formation of “cation-π” between Fe (III) and pyrene, which subsequently drove the pyrene removal process and accelerated the bacterial metabolic process. Moreover, a significant increase in the protein concentration, catechol dioxygenase (C12O and C23O) activities, and intracellular protein regulation in crude enzyme solution indicate a positive response of the bacteria during the iron ion-enhanced pyrene degradation process.