250 INDUCTION OF COLLAGENASE SECRETION IN HUMAN FIBROBLAST CULTURES BY GROWTH PROMOTING FACTORS
250 INDUCTION OF COLLAGENASE SECRETION IN HUMAN FIBROBLAST CULTURES BY GROWTH PROMOTING FACTORS
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[0400] 250通过生长促进因子诱导人成纤维细胞培养物中胶原酶的分泌
DOI：
10.1203/00006450-198504000-00280
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发表时间：
1985
影响因子：
3.6
通讯作者：
R. Ladda
中科院分区：
文献类型：
--
作者：
C. C. Chua;D. Geiman;G. Keller;R. Ladda
在人包皮成纤维细胞(HF)培养中，表皮生长因子、血小板衍生生长因子和转化生长因子(β−)诱导的两种蛋白质的Mr分别为60,000和55,000，约3.5−倍。用纯化的人成纤维细胞胶原酶抗体对诱导上清液进行免疫沉淀，鉴定为原胶原酶。经EGF处理的培养液中的胶原酶活性也被诱导为对照培养物的至少3倍。胶原酶的诱导依赖于蛋白质和RNA的从头合成，在加入EGF后10h即可观察到。虽然它们与不同的膜受体相互作用，但这三种生长因子诱导一种共同蛋白质的分泌。因此，我们的研究表明，胶原酶可能是细胞动员的重要中介，这一过程对有丝分裂是必不可少的。
In human foreskin fibroblast (HF) cultures, two proteins with Mr 60,000 and 55,000 were found to be induced about 3.5−fold by EGF, PDGF and β−TGF. The induced proteins were identified as procollagenases by immunoprecipitation of induced medium with antibodies to purified human fibroblast collagenase. Collagenase enzyme activity in the medium from EGF-treated cultures was also induced at least 3-fold compared to control cultures. Induction of collagenase was dependent upon de novo protein and RNA synthesis and was observed in the medium 10 hours after addition of EGF. Although they interact with separate membrane receptors, these three growth factors induce the secretion of a common protein. As a result, our study suggests that collagenase may be an important mediator of cell mobilization, a process which is essential for mito-genesis.