Cystathionine-β-synthase X proteins negatively regulate NADPH-thioredoxin reductase C activity
Cystathionine-β-synthase X proteins negatively regulate NADPH-thioredoxin reductase C activity
复制标题
胱硫醚-β-合酶 X 蛋白负向调节 NADPH-硫氧还蛋白还原酶 C 活性
DOI：
10.1016/j.bbrc.2023.02.055
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hisabori T.*
中科院分区：
文献类型：
--
作者：
Tran C.M.;Mihara S.;Yoshida K.*;Hisabori T.*
氧化还原调节是基于蛋白质巯基的氧化还原反应的翻译后修饰。硫氧还蛋白（Trx）是一种普遍存在的小分子蛋白质，在氧化还原调节中起着重要作用，但在植物叶绿体和一些蓝藻中也发现了一种独特的氧化还原调节因子，称为NADPH-Trx还原酶C（NTRC）。NTRC的几个重要功能已被提出，但控制NTRC活性的机制仍不确定。胱硫醚-β-合成酶X（CBSX）蛋白先前已显示与NTRC物理相互作用。在此基础上，本研究在体外对CBSX蛋白与拟南芥NTRC的相互作用进行了生化研究。因此，我们得出结论，CBSX蛋白作为负调节NTRC在AMP的存在下。
Redox regulation is a posttranslational modification based on the redox reaction of protein thiols. A small ubiquitous protein thioredoxin (Trx) plays a central role in redox regulation, but a unique redox-regulatory factor called NADPH-Trx reductase C (NTRC) is also found in plant chloroplasts and some cyanobacteria. Several important functions of NTRC have been suggested, but the mechanism for controlling NTRC activity remains undetermined. Cystathionine-β-synthase X (CBSX) proteins have been previously shown to interact with NTRC physically. Based on these observations, this study biochemically investigated the functional interaction between CBSX proteins and NTRC fromArabidopsis thalianain vitro. Consequently, we concluded that CBSX proteins act as negative regulators of NTRC in the presence of AMP.