Development of a serotype-specific DNA Microarray for identification of some Shigella and pathogenic Escherichia coli strains
Development of a serotype-specific DNA Microarray for identification of some Shigella and pathogenic Escherichia coli strains
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开发用于鉴定某些志贺氏菌和致病性大肠杆菌菌株的血清型特异性 DNA 微阵列
DOI：
10.1128/jcm.01389-06
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发表时间：
2006-12-01
影响因子：
9.4
通讯作者：
Wang, Lei
中科院分区：
文献类型：
--
作者：
Li, Yayue;Liu, Dan;Wang, Lei
志贺氏菌属和致病性大肠杆菌是人类传染病的主要原因，并且每年造成全世界数百万例腹泻。建立了一种简便、快速鉴定志贺菌和大肠杆菌高致病性血清型的方法。大肠杆菌是大规模流行病学研究、及时临床诊断和可靠的病原体检疫所必需的。本研究建立了以O型特异性基因为靶点的基因芯片，用于检测志贺菌和大肠杆菌15个血清型。大肠杆菌，包括宋内志贺菌;福氏志贺菌2a型;鲍氏志贺菌7、9、13、16和18型;大肠杆菌4、8和10型;和大肠杆菌。coli 055、O 111、O 114、O 128和O 157。对186株志贺菌和大肠杆菌的代表性菌株进行了检测。大肠杆菌0血清型，38个临床分离株，和9个菌株的其他细菌物种，通常存在于粪便样本中，并显示出特异性和可重复性。检测灵敏度为50 ng基因组DNA或10（4）CFU/ml的模拟粪便标本。这是首次报道志贺菌和致病性大肠杆菌的血清分型芯片。杆菌与传统的细菌培养法和抗血清凝集法相比，该方法具有许多优点，在基础微生物学研究、临床诊断、食品安全和流行病学监测等方面具有广阔的应用前景。
Shigella and pathogenic Escherichia coli are major causes of human infectious diseases and are responsible for millions of cases of diarrhea worldwide every year. A convenient and rapid method to identify highly pathogenic serotypes of Shigella and E. coli is needed for large-scale epidemiologic study, timely clinical diagnosis, and reliable quarantine of the pathogens. In this study, a DNA microarray targeting O-serotype-specific genes was developed to detect 15 serotypes of Shigella and E. coli, including Shigella sonnei; Shigella flexneri type 2a; Shigella boydii types 7, 9, 13, 16, and 18; Shigella dysenteriae types 4, 8, and 10; and E. coli 055, O111, O114, O128, and O157. The microarray was tested against 186 representative strains of all Shigella and E. coli 0 serotypes, 38 clinical isolates, and 9 strains of other bacterial species that are commonly present in stool samples and was shown to be specific and reproducible. The detection sensitivity was 50 ng genomic DNA or 10(4) CFU per ml in mock stool specimens. This is the first report of a microarray for serotyping Shigella and pathogenic E. coli. The method has a number of advantages over traditional bacterial culture and antiserum agglutination methods and is promising for applications in basic microbiological research, clinical diagnosis, food safety, and epidemiological surveillance.