Nephroprotective and antioxidant activities of ethyl acetate fraction of Euphorbia geniculata Ortega family Euphorbiaceae
Nephroprotective and antioxidant activities of ethyl acetate fraction of Euphorbia geniculata Ortega family Euphorbiaceae
复制标题
DOI：
10.1016/j.arabjc.2020.09.015
复制
发表时间：
2020-11-01
影响因子：
6
通讯作者：
Hassan, Hanaa M.
中科院分区：
文献类型：
--
作者：
Al-Yousef, Hanan M.;Alqahtani, Ali S.;Hassan, Hanaa M.
在大戟属植物研究的基础上，对大戟科（Euphorbiaceae）植物膝状大戟（EuphorbiageniculataOrtega（Eg））地上部分乙酸乙酯部位的化学成分进行了研究，分离鉴定了8种多酚类化合物;没食子酸（1），鞣花酸（2），槲皮素-3-O-芸香糖苷）（芸香苷）（3），槲皮素-3-O-吡喃葡萄糖苷-7-O-鼠李糖苷（4），槲皮素-3-O-鼠李糖苷（5）、槲皮素-3-O-β-D-吡喃葡萄糖苷（6）、槲皮素-3-O-β-D-阿拉伯糖苷（7）和槲皮素（8）。对总甲醇提取物中总黄酮和酚类物质的含量进行了定量测定。首次对乙酸乙酯部位进行了肾保护和抗氧化活性评价。采用硫代乙酰胺致大鼠肾损伤模型，观察银杏叶提取物对肾损伤的保护作用。抗氧化活性进行了评价，使用DPPH法。结果;定量估计总酚类化合物和黄酮含量的总甲醇提取物的银杏揭示高酚类化合物含量（285.4毫克GAE /克提取物）相比，黄酮含量（36.9毫克RE /克提取物）。八种多酚类化合物的分离与鉴定。用硫代乙酰胺作肾毒性剂，观察其肾保护活性。而预先给予Eg的乙酸乙酯组分可通过改变肾脏生物标志物，改善组织的氧化还原状态，使血清生化指标接近正常水平，从而显著减轻大鼠的肾毒性。结果表明，与抗坏血酸相比，银杏叶提取物具有明显的抗氧化活性。膝状花乙酸乙酯部位可作为天然来源的肾保护和抗氧化药物用于未来的治疗。(C)2020作者（S）由Elsevier B. V.代表沙特国王大学出版。
In continuation of our work on genus Euphorbia, the phytochemical investigation of ethyl acetate fraction of the aerial parts of Euphorbia geniculata Ortega (Eg) family Euphorbiaceae revealed the isolation and identification of eight polyphenolic compounds; gallic acid (1), ellagic acid (2), quercetin-3-O-rutinoside) (rutin) (3), quercetin-3-O-glucopyranoside-7-O-rhamnoside (4), quercetin-3-O-rhamnoside (5), quercetin-3-O-beta-D-glucopyranoside (6), and quercetin-3-O-beta-D-arabinoside (7) and quercetin (8) using spectrophotometric and physicochemical analysis. Quantitative estimation of total flavonoids and phenolics contents were carried out for total methanolic extract. Biological activities of ethyl acetate fraction including nephroprotective and antioxidant activities were evaluated for the first time. The nephroprotective potential of Eg was evaluated in male rats with thioacetamide induced kidney injury. Antioxidant activity was evaluated using DPPH method.Results; Quantitative estimation of total phenolics and flavonoids content in the total methanolic extract of Eg revealed high phenolics content (285.4 mg GAE /gm extract) in comparison with flavonoids content (36.9 mg RE /gm extract). Isolation and characterization of eight polyphenolic compounds. The nephroprotictive activity was studied using thioacetamide as nephrotoxicant agent resulted in marked nephrotoxicity. While pretreatment of rats with ethyl acetate fraction of Eg significantly attenuated the nephrotoxicity through alteration of kidney biomarkers, improving the redox status of the tissue and so brought the serum biochemical parameters nearly toward the normal levels. The study revealed significant antioxidant activity of Eg in comparison with ascorbic acid.Conclusion; The results suggested that E. geniculata Ortega ethyl acetate fraction could be used in future therapy as nephroprotective and antioxidant drugs of natural source. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.