BLADE-ON-PETIOLE1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
BLADE-ON-PETIOLE1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
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DOI：
10.1105/tpc.107.051938
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发表时间：
2007-06-01
期刊：
影响因子：
11.6
通讯作者：
Fletcher, Jennifer C.
中科院分区：
文献类型：
--
作者：
Ha, Chan Man;Jun, Ji Hyung;Fletcher, Jennifer C.
通过对异位表达BOP 1或BOP 2的功能缺失突变体和转基因植物的分析，我们报道了BOP 1和BOP 2在调节拟南芥侧器官细胞命运和极性中的新功能。35 S：B 0 P1和35 S：B 0 P2植物表现出非常短且紧凑的身材、下生叶和向下取向的长角果。我们发现，在35 S：BOP中，外侧器官边界（LOB）结构域基因不对称叶2（AS 2）和LOB上调，而在BOP突变体植物中下调。BOP 1或BOP 2的异位表达也导致I类knox基因表达的抑制。我们进一步证明了BOP 1和BOP 2在建立叶柄的近轴-远轴极性轴中的作用，在那里它们调节PHB和FIL的表达，并与AS 1和AS 2在功能上重叠。有趣的是，在这项研究中，我们发现KANADI 1（KAN 1）和KAN 2除了在促进远轴器官身份方面众所周知的作用之外，还起到促进近轴器官身份的作用。我们的数据表明，BOP 1和BOP 2作用于邻近侧向器官边界的细胞，以抑制赋予分生组织细胞命运的基因，并诱导促进侧向器官命运和极性的基因，从而限制器官形成细胞的发育潜力，并促进其分化。
We report a novel function for BLADE-ON-PETIOLE1 (BOP1) and BOP2 in regulating Arabidopsis thaliana lateral organ cell fate and polarity, through the analysis of loss-of-function mutants and transgenic plants that ectopically express BOP1 or BOP2. 35S: BOP1 and 35S: BOP2 plants exhibit a very short and compact stature, hyponastic leaves, and downward-orienting siliques. We show that the LATERAL ORGAN BOUNDARIES ( LOB) domain genes ASYMMETRIC LEAVES2 (AS2) and LOB are upregulated in 35S: BOP and downregulated in bop mutant plants. Ectopic expression of BOP1 or BOP2 also results in repression of class I knox gene expression. We further demonstrate a role for BOP1 and BOP2 in establishing the adaxial-abaxial polarity axis in the leaf petiole, where they regulate PHB and FIL expression and overlap in function with AS1 and AS2. Interestingly, during this study, we found that KANADI1 (KAN1) and KAN2 act to promote adaxial organ identity in addition to their well-known role in promoting abaxial organ identity. Our data indicate that BOP1 and BOP2 act in cells adjacent to the lateral organ boundary to repress genes that confer meristem cell fate and induce genes that promote lateral organ fate and polarity, thereby restricting the developmental potential of the organ-forming cells and facilitating their differentiation.