A mechanism of resistance to HIV-1 entry: Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages
A mechanism of resistance to HIV-1 entry: Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages
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DOI：
10.1006/viro.1999.9747
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发表时间：
1999-06-20
期刊：
影响因子：
3.7
通讯作者：
Broder, CC
中科院分区：
文献类型：
--
作者：
Dimitrov, DS;Norwood, D;Broder, CC
为了验证CXCR4与CD4和gp120的无效相互作用可能影响HIV-1进入的假设，我们用抗CXCR4的抗体将gp120和免疫共沉淀的CD4与巨噬细胞、单核细胞和淋巴细胞孵育。CD4在淋巴细胞和单核细胞中有效地共沉淀，而在巨噬细胞中不表达。巨噬细胞中CD4的过表达导致在恢复巨噬细胞融合敏感性的同时检测到CD4-CXCR4和gp120-CD4-CXCR4复合体。这些结果提示了一种抵抗某些X4 HIV-1毒株进入巨噬细胞的机制，以及一种解剖HIV进入初始阶段的方法。
To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.