Production of the Oocyte Maturation-Inducing Substance of Starfish by Heat Treatment of S-Adenosylmethionine
Production of the Oocyte Maturation-Inducing Substance of Starfish by Heat Treatment of S-Adenosylmethionine
复制标题
S-腺苷甲硫氨酸热处理生产海星卵母细胞成熟诱导物质
DOI：
10.2108/zsj.15.117
复制
发表时间：
1998
影响因子：
0.9
通讯作者：
Y. Nagahama
中科院分区：
文献类型：
--
作者：
M. Mita;I. Yasumasu;M. Saneyoshi;M. Yoshikuni;Y. Nagahama
摘要1-甲基腺嘌呤（1-MeA）是海星卵母细胞成熟诱导物质，但其生物合成机制尚不清楚。本研究表明，海星的MIS活性存在于s -腺苷蛋氨酸（SAM）热处理的反应物中。体外MIS的产生明显依赖于SAM溶液的温度：它随着温度的升高而增加，并在煮沸后5分钟内达到平台，尽管在低于20°C的孵育过程中几乎只观察到MIS。MIS的产生也依赖于溶液的ph。通过高效液相色谱和薄层色谱分析表明，MIS是1-MeA，尽管通过煮沸从SAM中获得的最大1-MeA量仅为初始SAM量的0.3%。此外，使用S-[14c -甲基]SAM表明1-MeA的甲基从SAM转移。因此，体内SAM有可能产生1-MeA。
Abstract 1-Methyladenine (1-MeA) has been identified as the oocyte maturation-inducing substance (MIS) in starfish, but little is known about its biosynthesis. This study showed that starfish MIS activity was present in a reactant derived from S-adenosylmethionine (SAM) by heat treatment. In vitro MIS production was markedly dependent on the temperature of the SAM solution: it increased as the temperature was raised, and reached a plateau within 5 min upon boiling, although hardly only MIS was observed upon incubation below 20°C. MIS production was also dependent on the solution pH. Analyses by high-performance liquid chromatography and thin-layer chromatography showed that the MIS was 1-MeA, though the maximum amount of 1-MeA obtained from SAM by boiling was only 0.3% of the initial SAM amount. Furthermore, use of S-[14C-methyl]SAM showed that a methyl group of 1-MeA was transferred from the SAM. Thus, it is possible that 1-MeA may be produced from SAM in vivo.