Peripheral nerve regeneration in the MRL/MpJ ear wound model
Peripheral nerve regeneration in the MRL/MpJ ear wound model
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DOI：
10.1111/j.1469-7580.2010.01313.x
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发表时间：
2011-02-01
影响因子：
2.4
通讯作者：
Ferguson, Mark W. J.
中科院分区：
文献类型：
--
作者：
Buckley, Gemma;Metcalfe, Anthony D.;Ferguson, Mark W. J.
MRL/MPJ小鼠表现出加速愈合耳部穿孔伤口而不形成疤痕的能力(而躯干背部表面的伤口愈合时有疤痕形成)，为研究成年哺乳动物的组织再生提供了难得的机会。形成类似胚芽的结构，随后修复受伤耳朵的结构，包括软骨、皮肤、毛囊和脂肪组织。我们试图评估MRL/MPJ株是否也具有增强的周围神经再生能力。雌性MRL/MPJ和C57BL/6小鼠每只耳中央分别用2 mm的切除活检针和背部皮肤的2 mm切除活检针致伤。用免疫组织化学泛神经丝和CD31标记双重染色观察躯干和耳部创面的神经再支配和血管再生。在伤后10~21d，当胚芽样结构形成时，MRL/MPJ小鼠耳的再生神经密度显著高于C57BL/6(P>；0.01)。与背部皮肤伤口不同，耳部伤口的神经再生先于血管再生，重述了哺乳动物的早期发育。免疫组织化学数据表明，胚芽间充质内的因子，如聚集素，可能会引导再生的耳朵组织中的神经再生。
The MRL/MpJ mouse displays an accelerated ability to heal ear punch wounds without scar formation (whereas wounds on the dorsal surface of the trunk heal with scar formation), offering a rare opportunity for studying tissue regeneration in adult mammals. A blastema-like structure develops and subsequently the structure of the wounded ear is restored, including cartilage, skin, hair follicles and adipose tissue. We sought to assess if the MRL/MpJ strain also possessed an enhanced capacity for peripheral nerve regeneration. Female MRL/MpJ and C57BL/6 mice were wounded with a 2-mm excisional biopsy punch to the centre of each ear and two 4-mm excisional biopsy punches to the dorsal skin. Immunohistochemical dual staining of pan-neurofilament and CD31 markers was used to investigate reinnervation and vascularisation of both the dorsal surface of the trunk and ear wounds. The MRL/MpJ mouse ear exhibited a significantly (P > 0.01) higher density of regenerated nerves than C57BL/6 between 10 and 21 days post-wounding when the blastema-like structure was forming. Unlike dorsal skin wounds, nerve regeneration in the ear wound preceded vascularisation, recapitulating early mammalian development. Immunohistochemical data suggest that factors within the blastemal mesenchyme, such as aggrecan, may direct nerve regrowth in the regenerating ear tissue.