An Arabidopsis dynamin-related protein, DRP3A, controls both peroxisomal and mitochondrial division
An Arabidopsis dynamin-related protein, DRP3A, controls both peroxisomal and mitochondrial division
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DOI：
10.1111/j.1365-313x.2004.02063.x
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发表时间：
2004-05-01
期刊：
影响因子：
7.2
通讯作者：
Nishimura, M
中科院分区：
文献类型：
--
作者：
Mano, S;Nakamori, C;Nishimura, M
过氧化物酶体在细胞内的大小、形状、数量和位置发生巨大变化，但过氧化物酶体的分裂过程尚未被表征。我们筛选了许多具有异常过氧化物酶体形态的拟南芥突变体（apm突变体）。在其中一种突变体 apm1 中，过氧化物酶体很长且数量减少，显然是由于分裂受到抑制。我们发现 APM1 编码动力相关蛋白 3A (DRP3A)，并且 APM1/DRP3A 的突变也会导致线粒体形态异常。瞬时表达分析表明DRP3A与过氧化物酶体的胞质侧相关。这些发现表明，高等植物中的过氧化物酶体和线粒体分裂涉及相同的动力分子。我们还报道，拟南芥的生长需要多种细胞器（包括过氧化物酶体和线粒体）的合作，在apm1中受到抑制，这表明过氧化物酶体和线粒体的形态变化降低了这些细胞器的代谢效率。
Peroxisomes undergo dramatic changes in size, shape, number, and position within the cell, but the division process of peroxisomes has not been characterized. We screened a number of Arabidopsis mutants with aberrant peroxisome morphology (apm mutants). In one of these mutants, apm1, the peroxisomes are long and reduced in number, apparently as a result of inhibition of division. We showed that APM1 encodes dynamin-related protein 3A (DRP3A), and that mutations in APM1/DRP3A also caused aberrant morphology of mitochondria. The transient expression analysis showed that DRP3A is associated with the cytosolic side of peroxisomes. These findings indicate that the same dynamin molecule is involved in peroxisomal and mitochondrial division in higher plants. We also report that the growth of Arabidopsis, which requires the cooperation of various organelles, including peroxisomes and mitochondria, is repressed in apm1, indicating that the changes of morphology of peroxisomes and mitochondria reduce the efficiency of metabolism in these organelles.