Androstenediol-induced restoration of responsiveness to influenza vaccination in mice.
Androstenediol-induced restoration of responsiveness to influenza vaccination in mice.
复制标题
雄烯二醇诱导小鼠对流感疫苗反应的恢复。
DOI：
10.1093/gerona/55.9.b418
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Sheridan,JF
中科院分区：
文献类型：
--
作者：
Padgett,DA;MacCallum,RC;Loria,RM;Sheridan,JF
雄烯二醇（AED）是脱氢表雄酮（DHEA）的代谢产物，调节先天性和适应性免疫反应。为了检查AED是否可以有效地逆转与年龄相关的抗病毒免疫力下降，3个月、10个月和22个月大的小鼠在接种疫苗前10天开始用AED硫酸盐（AED-S）治疗45天。随后，用次优剂量的市售三价流感疫苗对小鼠进行致敏和加强免疫。在疫苗接种期间用AED-S处理10月龄动物，可将循环抗病毒免疫球蛋白G的滴度提高至与3月龄小鼠相当的水平。此外，AED-S治疗保护10月龄动物在二次接种后21天免受致死剂量流感病毒的鼻内攻击。虽然AED-S治疗22个月大的小鼠并没有增强疫苗应答，也没有保护小鼠免受致命攻击，但10个月大的动物的数据表明，AED-S治疗可以预防免疫衰老的早期表现。
Androstenediol (AED), a metabolite of dehydroepiandrosterone (DHEA) regulates innate and adaptive immune responses. To examine whether AED could effectively reverse the age-associated decline of antiviral immunity, 3-, 10-, and 22-month-old mice were treated with AED-sulfate (AED-S) for 45 days beginning 10 days prior to vaccination. Subsequently, mice were primed and boosted with suboptimal doses of a commercially-available trivalent influenza vaccine. Treatment of 10-month-old animals with AED-S during vaccination increased the titer of circulating antiviral immunoglobulin G to levels comparable with those in 3-month-old mice. Furthermore, AED-S treatment protected 10-month-old animals from intranasal challenge with a lethal dose of influenza virus 21 days after secondary vaccination. Although AED-S treatment of 22-month-old mice did not enhance vaccine responses and failed to protect against lethal challenge, the data from the 10-month-old animals suggest that treatment with AED-S will prevent the early manifestations of immunosenescence.