Development of dimethyl sulfoxide biosensor using a mediator immobilized enzyme electrode
Development of dimethyl sulfoxide biosensor using a mediator immobilized enzyme electrode
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作者：
Cheng, HC;Abo, M;Okubo, A
以二甲基亚砜还原酶（DMSO- r）为载体构建了固定化二甲基亚砜（DMSO）传感器。以甲基紫素（MV）为介体，用Nafion聚合物固定在玻碳（GC）电极上。改性气相色谱电极上的透析膜保留了球形红杆菌反硝化菌的DMSO-R。观察了DMSO对电流信号的响应。DMSO的校准曲线线性范围为0 ~ 600 μ m。在200 muM DMSO条件下，反应时间在100 s以内，相对标准偏差为4% （n = 4）。为了消除样品中氧气产生的背景噪声，葡萄糖氧化酶-过氧化氢酶保留DMSO传感器也进行了检测。
A mediator immobilized dimethyl sulfoxide (DMSO) sensor using DMSO reductase (DMSO-R) was constructed. Methyl viologen (MV) was used as a mediator and immobilized on a glassy carbon (GC) electrode with Nafion polymer. DMSO-R from Rhodobacter sphaeroides f. sp. denitrificans was retained by a dialysis film on the modified GC electrode. The amperometric signal in response to DMSO was observed. The linear range of the calibration curve for DMSO was between 0 and 600 muM. The response time was within 100 s and the relative standard deviation was 4% at 200 muM DMSO (n = 4). To eliminate the background noise derived from oxygen in samples, the glucose oxidase-catalase retained DMSO sensor was also examined.