DNA transfection using linear poly(ethylenimine) prepared by controlled acid hydrolysis of poly(2-ethyl-2-oxazoline)
DNA transfection using linear poly(ethylenimine) prepared by controlled acid hydrolysis of poly(2-ethyl-2-oxazoline)
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DOI：
10.1016/s0168-3659(01)00310-8
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发表时间：
2001-06-15
影响因子：
10.8
通讯作者：
Park, TG
中科院分区：
文献类型：
--
作者：
Jeong, JH;Song, SH;Park, TG
以聚（2-乙基-2-恶唑啉）（PEtOz）为原料，通过控制水解制备了一系列主链上含有不同阳离子电荷密度的线型聚乙烯亚胺（L-PEI），并将其用作非病毒DNA转染剂。研究了L-PEI阳离子电荷密度和分子量对细胞毒性和转染效率的影响。通过使用荧光素酶报告基因系统监测转染效率。凝胶阻滞实验和动态光散射实验表明，L-PEI的凝聚能力适合转染。获得了高度致密的L-PEI/DNA复合物（类似于150 nm），表面电荷值约为+28.4 mV。细胞毒性在很大程度上受L-PEI的水解百分比以及分子量的影响。荧光素酶质粒DNA对NIH 3 T3成纤维细胞的转染效率在很大程度上取决于聚合物主链中的水解百分比（电荷密度）和L-PEI的分子量，但与用于DNA缩合的阳离子电荷的总量无关。水解率为88.0%的L-PEI表现出与常用的支化PEI相当的转染效率。(C)2001 Elsevier Science B. V.保留所有权利。
A series of linear poly(ethylenimine) (L-PEI) containing varying amounts of cationic charge density in its backbone was produced by controlled hydrolysis of poly(2-ethyl-2-oxazoline) (PEtOz) for using as a nonviral DNA transfection agent. The effects of cationic charge density and molecular weight of the L-PEI on the cytotoxicity and transfection efficiency were studied. The efficiency of transfection was monitored by using a luciferase reporter gene system. Gel retardation assay and dynamic light scattering (DLS) showed that the condensation capacity of L-PEI was suitable for transfection. Highly compacted L-PEI/DNA complex (similar to 150 nm) was obtained with a surface charge value of around +28.4 mV. Cell cytotoxicity was affected to a great extent by the hydrolysis percent of L-PEI as well as by the molecular weight. Transfection efficiency of luciferase plasmid DNA against NIH 3T3 fibroblast was largely dependent upon the hydrolysis percent (charge density) in the polymer backbone and the molecular weight of the L-PEI, but independent of the total amount of cationic charges used for DNA condensation. L-PEI with a hydrolysis percent of 88.0% exhibited comparable transfection efficiency to that of commonly used branched PEI. (C) 2001 Elsevier Science B.V. All rights reserved.