Expression of a putative alfalfa helicase increases tolerance to abiotic stress in Arabidopsis by enhancing the capacities for ROS scavenging and osmotic adjustment
Expression of a putative alfalfa helicase increases tolerance to abiotic stress in Arabidopsis by enhancing the capacities for ROS scavenging and osmotic adjustment
复制标题
假定的苜蓿解旋酶的表达通过增强ROS清除和渗透调节的能力来增加拟南芥对非生物胁迫的耐受性
DOI：
10.1016/j.jplph.2008.06.018
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Zhang, Xian Sheng
中科院分区：
文献类型：
--
作者：
Luo, Yan;Liu, Yu Bo;Zhang, Xian Sheng
已知植物解旋酶参与盐度和低温耐受性。不过，功能性的。解旋酶在植物抗氧化反应中的参与尚未被描述。我们从苜蓿（紫花苜蓿）中分离出包含 DEAD-box 的 cDNA 序列，它是豌豆 DNA 解旋酶 45 (PDH45) 的同源物，并将其命名为苜蓿解旋酶 1 (MH1)。 35S::MH1-GFP 瞬时转染洋葱表皮显示 MH1 定位于细胞核。在苜蓿的根、茎和叶中检测到MH1的表达。此外，实时PCR分析显示甘露醇、氯化钠、甲基紫精和脱落酸诱导MH1的表达。拟南芥中 MH1 的异位表达改善了干旱、盐和氧化胁迫下的种子萌发和植物生长。转基因拟南芥植物的渗透调节能力、超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性以及果仁糖含量也有所提高。我们的结果表明，MH1 对活性氧 (ROS) 做出反应，并通过增强 ROS 清除和渗透调节的能力来耐受干旱和盐胁迫。 (C) 2008 爱思唯尔有限公司。版权所有。
Plant helicases are known to be involved in salinity and low-temperature tolerance. However, a functional. involvement of helicases in the antioxidative response of plants has not been described. We have isolated a DEAD-box-containing cDNA sequence from Medicago sativa (alfalfa) that is a homolog of the pea DNA helicase 45 (PDH45) and named it M. sativa helicase 1 (MH1). Transient transfection of 35S::MH1-GFP to onion epidermis revealed that MH1 was Localized in the nucleus. Expression of MH1 was detected in roots, stems and Leaves of alfalfa. Furthermore, real-time PCR analysis revealed that mannitol, NaCl, methyl viologen and abscisic acid induced the expression of MH1. The ectopic expression of MH1 in Arabidopsis improved seed germination and plant growth under drought, salt and oxidative stress. The capacity for osmotic adjustment, superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities and praline content were also elevated in the transgenic Arabidopsis plants. Our results suggest that MH1 responds to reactive oxygen species (ROS) and functions in drought and salt stress tolerance by enhancing the capacities for ROS scavenging and osmotic adjustment. (C) 2008 Elsevier GmbH. All rights reserved.