Gold Nanoparticle-Enabled Real-Time Ligation Chain Reaction for Ultrasensitive Detection of DNA
Gold Nanoparticle-Enabled Real-Time Ligation Chain Reaction for Ultrasensitive Detection of DNA
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DOI：
10.1021/ja306265n
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发表时间：
2012-09-12
影响因子：
15
通讯作者：
Gao, Zhiqiang
中科院分区：
文献类型：
--
作者：
Shen, Wei;Deng, Huimin;Gao, Zhiqiang
基于对结扎链反应（LCR）产物的检测和使用金纳米颗粒（AuNPs）作为信号发生器，开发了一种简单、超灵敏的比色DNA测定方法。在LCR过程中，aunp被连接在一起，在足够数量的热循环后实时产生明显的颜色变化。该方案的累积性质产生了20 aM的检测限，选择性因子为10(3)。
A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).