miR-122-5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)
miR-122-5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A)
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DOI：
10.1002/2211-5463.12730
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发表时间：
2019-09-29
期刊：
影响因子：
2.6
通讯作者：
Zhang, Shu-Mao
中科院分区：
文献类型：
--
作者：
Ding, Feng-Na;Gao, Bao-Hong;Zhang, Shu-Mao
宫颈癌是全球最常见的妇科恶性肿瘤之一，不幸的是，放疗和化疗对某些病例的治疗效果不佳，5年生存率仅为40- 50%。细胞分裂周期25 A（CDC 25 A）已被证明在多种肿瘤细胞中诱导辐射抗性，但CDC 25 A在宫颈癌的辐射抗性中的作用尚未完全阐明。在此，我们报道了CDC 25 A在宫颈癌组织和细胞中高表达，而miR-122- 5 p低表达。利用TargetScan数据库预测miR-122- 5 p作用的靶位为CDC 25 A，并通过Western blot、real-time PCR和双荧光素酶报告基因分析进一步验证miR-122- 5 p与CDC 25 A的相互作用。在X射线照射下，上调CDC 25 A表达可增强宫颈癌细胞的放射抵抗力，而过表达miR-122- 5 p或敲低CDC 25 A则抑制宫颈癌细胞集落的存活并诱导其凋亡。总之，我们的数据表明，miR-122- 5 p通过靶向CDC 25 A增强宫颈癌细胞的放射敏感性。
Cervical cancer is one of the most common gynecological malignancies globally, Unfortunately, radiotherapy and chemotherapy are not effective at treating some cases of this disease, and the 5-year survival rate is only 40-50%. Cell division cycle 25A (CDC25A) has been shown to induce radioresistance in a variety of tumor cells, but the role of CDC25A in the radioresistance of cervical cancer has not been fully elucidated. Here, we report that CDC25A is highly expressed and miR-122-5p lowly expressed in cervical cancer tissues and cells. The TargetScan database was used to predict CDC25A as a target of miR-122-5p, and the interactions between miR-122-5p and CDC25A were further confirmed by western blot, real-time PCR and dual-luciferase reporter assay. Under X-ray irradiation, up-regulation of CDC25A can promote the radiation resistance of cervical cancer cells, whereas overexpression of miR-122-5p or knockdown of CDC25A inhibits the survival and induces apoptosis of cervical cancer colonies. In conclusion, our data suggest that miR-122-5p enhances the radiosensitivity of cervical cancer cells by targeting CDC25A.