HIGH BASAL EXPRESSION AND 3,5,3'-TRIIODOTHYRONINE REGULATION OF MESSENGER RIBONUCLEIC-ACID S14 IN LIPOGENIC TISSUES
HIGH BASAL EXPRESSION AND 3,5,3'-TRIIODOTHYRONINE REGULATION OF MESSENGER RIBONUCLEIC-ACID S14 IN LIPOGENIC TISSUES
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DOI：
10.1210/endo-117-6-2259
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发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
OPPENHEIMER, JH
中科院分区：
文献类型：
--
作者：
JUMP, DB;OPPENHEIMER, JH
我们最近报道，T3和高碳水化合物生脂饮食引起大鼠肝脏胞浆蛋白点14(17,010molwt；4.9pI)编码的mRNA迅速且显著增加。通过杂交实验，我们已经证明了肝脏mRNAS14对T3和饮食调控的反应类似于许多肝脏造脂酶的反应。我们通过检测mRNAS14在其他生脂和非生脂组织中的表达和调控来扩展我们之前的研究。MRNAS14在3个脂肪库(附睾、腹膜后和棕色脂肪)和哺乳期乳腺中的相对表达水平分别是正常甲状腺男性肝脏的10倍和4倍。RRNAS14在肝脏、附睾脂和乳腺中的表达在T3时增强，而饲喂生脂饲料仅在肝脏和脂肪中增加mRNAS14的表达。相反，在各种非致脂组织(脑、心、肾、肺、脾、睾丸和垂体)中，mRNAS14的相对水平是肝脏的7%或更低。饮食和甲状腺状况都不影响非生脂组织中mRNAS14的水平。我们还发现，15日龄大鼠的肝脏mRNA514的表达水平是成年(2月龄)肝脏水平的0.3%，从15日龄到30日龄增加了186倍。我们推测，新生大鼠肝脏中mRNA514的低水平可能是由于牛奶饮食中的高脂肪所致。在成脂组织中mRNAS14的高基础水平的存在以及T3和饮食对其的调节表明，Spot 14蛋白可能在能量生产中所用的脂肪的合成、代谢、运输或储存的某些方面发挥重要作用。此外，我们的结果强调，组织中T3受体的存在不足以赋予T3对mRNAS14表达的调节。调节14号点基因还需要额外的组织特异性因子。
We recently reported that T3 and the high carbohydrate lipogenic diet elicit a brisk and marked increase in the rat liver mRNA coding for the cytosolic protein Spot 14 (17,010 mol wt; 4.9 pI). By means of a hybridization assay, we have shown that the response of hepatic mRNAS14 to T3 and dietary manipulation is analogous to the response of many hepatic lipogenic enzymes. We extend our previous studies by examining mRNAS14 expression and regulation in other lipogenic and nonlipogenic tissues. The relative levels of mRNAS14 expression in 3 fat depots (epididymal, retroperitoneal, and brown fat) and lactating mammary gland are 10- and 4-fold higher, respectively, than that in euthyroid male liver. Expression of mRNAS14 in liver, epididymal fat, and mammary gland is augmented by T3, whereas feeding a lipogenic diet augments mRNAS14 in liver and fat only. In contrast, the relative levels of mRNAS14 in various nonlipogenic tissues (brain, heart, kidney, lung, spleen, testes, and pituitary) are 7% that in liver or less. Neither diet nor thyroid status influenced mRNAS14 levels in the nonlipogenic tissues. We also found that hepatic mRNA514 in the 15-day-old rat is expressed at 0.3% of the adult (2-month) hepatic level and increases 186-fold from 15 to 30 days of age. We speculate that low levels of mRNA514 in neonatal rat liver may be due to high fat in the milk diet. The presence of high basal levels of mRNAS14 in lipogenic tissues and its regulation by T3 and diet suggest that the Spot 14 protein may play an important role in some aspect of synthesis, metabolism, transport, or storage of lipid used in energy production. Further, our results emphasize that the presence of T3 receptors in a tissue is not sufficient to confer T3 regulation of mRNAS14 expression. Additional tissue-specific factors are required for the regulation of the Spot 14 gene.