Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats
Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats
复制标题
超顺磁性氧化铁（SPIO）纳米颗粒标记的内皮祖细胞（EPC）给药抑制大鼠异位骨化
DOI：
10.1016/j.nano.2019.102078
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Zhong Biao
中科院分区：
文献类型：
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作者：
Zhang Chi;Hsu Peichun;Wang Dimin;Zhang Wei;Zhang Changqing;Guo Shang;Yang Weichao;Wei Xiaokang;Zhang Yang;Zhong Biao
异位骨化（HO）是一种疼痛性疾病，其特征是损伤后在骨骼外异位形成不需要的骨。SPIO纳米粒子治疗已广泛应用于各种骨科疾病。然而，SPIO纳米颗粒对异位骨化的影响仍然未知。本研究制备了SPIO纳米粒，并在大鼠模型中评价了其对HO的作用机制。结果显示，含有SMAD 7的SPIO纳米颗粒处理导致体外上皮-间充质转化（EMT）相关蛋白表达的降低。此外，SPIO纳米颗粒标记的EPCs移植有效地防止异位骨化和抑制内皮间质转化（EndMT）在HO大鼠。此外，SPIO纳米颗粒标记的EPCs移植抑制HO大鼠胚胎成纤维细胞（EFs）的成骨和成脂分化。我们的研究结果表明，给予SPIO纳米颗粒标记的EPCs可以抑制大鼠的异位骨化，这可能是一种潜在的治疗方法，用于医疗干预治疗HO在临床上。
Heterotopic ossification (HO) is a painful disease characterized by unwanted bone ectopic formation outside of the skeleton after injury. SPIO nanoparticles therapy has been widely used in diverse orthopedic diseases. However, the effect of SPIO nanoparticles on heterotopic ossification remains unknown. Here, we prepared the SPIO nanoparticles carrying mothers against decapentaplegic homolog 7 (SMAD7) and evaluated their mechanism function to HO in a rat model. The results revealed that SPIO nanoparticles containing SMAD7 treatment lead to a decrease in epithelial-mesenchymal transition (EMT) relevant protein expression in vitro. Moreover, SPIO nanoparticles labeled EPCs transplantation effectively prevented heterotopic ossification and inhibited endothelial-mesenchymal transition (EndMT) in HO rats. In addition, SPIO nanoparticles labeled EPCs transplantation suppressed osteogenic and adipogenic differentiation of embryonic fibroblasts (EFs) in HO rats. Our results demonstrated that administration of SPIO nanoparticles labeled EPCs could inhibit heterotopic ossification in rats, which might be a potential therapy method for a medical intervention to treat HO in clinic.