The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner
The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner
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DOI：
10.1093/nar/24.9.1608
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发表时间：
1996-05-01
影响因子：
14.9
通讯作者：
Buckle, M
中科院分区：
文献类型：
--
作者：
Brunel, F;Zakin, MM;Buckle, M
多聚嘧啶束结合蛋白（PTB）是一种功能尚不清楚的细胞因子。各种RNA或单链DNA序列已显示与PTB相互作用。在本文中，使用激光紫外交联和电泳迁移率变动分析探针DNA-蛋白质相互作用，我们证明，PTB结合在一个单链DNA靶是高度序列特异性的。我们提供的数据显示，PTB与腺病毒主要晚期启动子转录起始子的顶链相互作用，该序列富含嘧啶残基。我们还表明，PTB被组织成至少两个不同的结合域。
Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV cross-linking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.