Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
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DOI：
10.1016/j.femsle.2004.03.015
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发表时间：
2004-05-01
影响因子：
2.1
通讯作者：
Nishimura, K
中科院分区：
文献类型：
--
作者：
Endo, S;Komori, T;Nishimura, K
副球孢子菌病是一种深部真菌病，由热依赖性二形性真菌巴西副球孢子菌引起，在拉丁美洲国家流行。我们通过环介导等温扩增 (LAMP) 在 22 个临床分离株和 7 个犰狳衍生分离株中检测到巴西 P. brasiliensis 的物种特异性 gp43 基因。扩增的 DNA 呈梯状，具有特定的条带图案。 LAMP方法的优点是速度快；鉴定微生物和诊断疾病只需要3小时。我们还从副球孢子菌病石蜡包埋组织样本中提取的 DNA 获得了阳性结果，表明该方法可能在不久的将来实现临床应用。 (C) 2004 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. The amplified DNA appeared as a ladder with a specific banding pattern. The advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.